An optimized protocol for the extraction and quantification of cytosolic DNA in mammalian cells

An optimized protocol for the extraction and quantification of cytosolic DNA in mammalian cells

Summary: Leakage of mitochondrial or nuclear DNA into the cytosol can occur following viral infections, radiation damage, and some cancers. Here, we present an optimized protocol for isolating and quantifying cytosolic DNA from mammalian cells. We describe steps for collecting cytosolic fractions fr...

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Detalles Bibliográficos
Autores principales: Aminu S. Jahun, Frederic Sorgeloos, Ian G. Goodfellow
Formato: Artículo
Lenguaje:English
Publicado: Elsevier 2024-03-01
Colección:STAR Protocols
Materias:
Cancer
Cell Biology
Cell separation/fractionation
Cell-based Assays
Immunology
Molecular Biology
Acceso en línea:http://www.sciencedirect.com/science/article/pii/S2666166724000789

Internet

http://www.sciencedirect.com/science/article/pii/S2666166724000789

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