An optimized protocol for the extraction and quantification of cytosolic DNA in mammalian cells

An optimized protocol for the extraction and quantification of cytosolic DNA in mammalian cells

Summary: Leakage of mitochondrial or nuclear DNA into the cytosol can occur following viral infections, radiation damage, and some cancers. Here, we present an optimized protocol for isolating and quantifying cytosolic DNA from mammalian cells. We describe steps for collecting cytosolic fractions fr...

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Bibliographic Details
Main Authors: Aminu S. Jahun, Frederic Sorgeloos, Ian G. Goodfellow
Format: Article
Language:English
Published: Elsevier 2024-03-01
Series:STAR Protocols
Subjects:
Cancer
Cell Biology
Cell separation/fractionation
Cell-based Assays
Immunology
Molecular Biology
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166724000789

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