Protocol to isolate and enrich mouse splenic naive CD4+ T cells for in vitro CD4+CD8αα+ cell induction
Summary: Recent studies have shown that CD4+CD8αα+ T cells are induced in the hypoxic environment of the small intestinal epithelium. Herein, we describe a protocol for CD4+CD8αα+ T cell induction from freshly isolated naive CD4+ T cells, including procedures for the isolation and enrichment of mous...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2022-12-01
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| Series: | STAR Protocols |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666166722006086 |