A catalogue of biochemically diverse CRISPR-Cas9 orthologs

A catalogue of biochemically diverse CRISPR-Cas9 orthologs

A few bacterial Cas9 nucleases have been repurposed as genome editing tools. Here, the authors use bioinformatic and biochemical analyses to characterize 79 Cas9 proteins, revealing substantial functional diversity and thus expanding the available toolbox of RNA-programmable CRISPR-associated nuclea...

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Bibliographic Details
Main Authors: Giedrius Gasiunas, Joshua K. Young, Tautvydas Karvelis, Darius Kazlauskas, Tomas Urbaitis, Monika Jasnauskaite, Mantvyda M. Grusyte, Sushmitha Paulraj, Po-Hao Wang, Zhenglin Hou, Shane K. Dooley, Mark Cigan, Clara Alarcon, N. Doane Chilcoat, Greta Bigelyte, Jennifer L. Curcuru, Megumu Mabuchi, Zhiyi Sun, Ryan T. Fuchs, Ezra Schildkraut, Peter R. Weigele, William E. Jack, G. Brett Robb, Česlovas Venclovas, Virginijus Siksnys
Format: Article
Language:English
Published: Nature Portfolio 2020-11-01
Series:Nature Communications
Online Access:https://doi.org/10.1038/s41467-020-19344-1

Internet

https://doi.org/10.1038/s41467-020-19344-1

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