Probing the active site of Class 3 L-asparaginase by mutagenesis. I. Tinkering with the zinc coordination site of ReAV

Probing the active site of Class 3 L-asparaginase by mutagenesis. I. Tinkering with the zinc coordination site of ReAV

ReAV, the inducible Class-3 L-asparaginase from the nitrogen-fixing symbiotic bacterium Rhizobium etli, is an interesting candidate for optimizing its enzymatic potential for antileukemic applications. Since it has no structural similarity to known enzymes with this activity, it may offer completely...

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Bibliographic Details
Main Authors: Kinga Pokrywka, Marta Grzechowiak, Joanna Sliwiak, Paulina Worsztynowicz, Joanna I. Loch, Milosz Ruszkowski, Miroslaw Gilski, Mariusz Jaskolski
Format: Article
Language:English
Published: Frontiers Media S.A. 2024-04-01
Series:Frontiers in Chemistry
Subjects:
hydrolase
amidohydrolase
L-asparaginase
leukemia
metalloprotein
site-directed mutagenesis
Online Access:https://www.frontiersin.org/articles/10.3389/fchem.2024.1381032/full

Internet

https://www.frontiersin.org/articles/10.3389/fchem.2024.1381032/full

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