Fresh Versus Frozen Stool for Fecal Microbiota Transplantation—Assessment by Multimethod Approach Combining Culturing, Flow Cytometry, and Next-Generation Sequencing

Fresh Versus Frozen Stool for Fecal Microbiota Transplantation—Assessment by Multimethod Approach Combining Culturing, Flow Cytometry, and Next-Generation Sequencing

The objective of this work was to compare the quality of FMT preparations made from fresh feces with those made from feces frozen at –30°C without any pre-processing or cryopreservation additives. The research hypothesis was that such preservation protocol (frozen whole stool, then thawed and proces...

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Bibliographic Details
Main Authors: Jaroslaw Bilinski, Mikolaj Dziurzynski, Pawel Grzesiowski, Edyta Podsiadly, Anna Stelmaszczyk-Emmel, Tomasz Dzieciatkowski, Karol Lis, Martyna Tyszka, Krzysztof Ozieranski, Łukasz Dziewit, Grzegorz W. Basak
Format: Article
Language:English
Published: Frontiers Media S.A. 2022-07-01
Series:Frontiers in Microbiology
Subjects:
fecal microbiota transplantation
conservation
gut microbiota
culturing
next-generation sequencing
flow cytometry
Online Access:https://www.frontiersin.org/articles/10.3389/fmicb.2022.872735/full

Internet

https://www.frontiersin.org/articles/10.3389/fmicb.2022.872735/full

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