Pure lignin induces overexpression of cytochrome P450 (CYP) encoding genes and brings insights into the lignocellulose depolymerization by Trametes villosa
Trametes villosa is a remarkable white-rot fungus (WRF) with the potential to be applied in lignocellulose conversion to obtain chemical compounds and biofuels. Lignocellulose breakdown by WRF is carried out through the secretion of oxidative and hydrolytic enzymes. Despite the existing knowledge ab...
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Elsevier
2024-04-01
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author | Luiz Marcelo Ribeiro Tomé Mariana Teixeira Dornelles Parise Doglas Parise Vasco Ariston de Carvalho Azevedo Bertram Brenig Fernanda Badotti Aristóteles Góes-Neto |
author_facet | Luiz Marcelo Ribeiro Tomé Mariana Teixeira Dornelles Parise Doglas Parise Vasco Ariston de Carvalho Azevedo Bertram Brenig Fernanda Badotti Aristóteles Góes-Neto |
author_sort | Luiz Marcelo Ribeiro Tomé |
collection | DOAJ |
description | Trametes villosa is a remarkable white-rot fungus (WRF) with the potential to be applied in lignocellulose conversion to obtain chemical compounds and biofuels. Lignocellulose breakdown by WRF is carried out through the secretion of oxidative and hydrolytic enzymes. Despite the existing knowledge about this process, the complete molecular mechanisms involved in the regulation of this metabolic system have not yet been elucidated. Therefore, in order to understand the genes and metabolic pathways regulated during lignocellulose degradation, the strain T. villosa CCMB561 was cultured in media with different carbon sources (lignin, sugarcane bagasse, and malt extract). Subsequently, biochemical assays and differential gene expression analysis by qPCR and high-throughput RNA sequencing were carried out. Our results revealed the ability of T. villosa CCMB561 to grow on lignin (AL medium) as the unique carbon source. An overexpression of Cytochrome P450 was detected in this medium, which may be associated with the lignin O-demethylation pathway. Clusters of up-regulated CAZymes-encoding genes were identified in lignin and sugarcane bagasse, revealing that T. villosa CCMB561 acts simultaneously in the depolymerization of lignin, cellulose, hemicellulose, and pectin. Furthermore, genes encoding nitroreductases and homogentisate-1,2-dioxygenase that act in the degradation of organic pollutants were up-regulated in the lignin medium. Altogether, these findings provide new insights into the mechanisms of lignocellulose degradation by T. villosa and confirm the ability of this fungal species to be applied in biorefineries and in the bioremediation of organic pollutants. |
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spelling | doaj.art-f01ce11b357c40d285bfea09ad55c5a32024-03-28T06:38:26ZengElsevierHeliyon2405-84402024-04-01107e28449Pure lignin induces overexpression of cytochrome P450 (CYP) encoding genes and brings insights into the lignocellulose depolymerization by Trametes villosaLuiz Marcelo Ribeiro Tomé0Mariana Teixeira Dornelles Parise1Doglas Parise2Vasco Ariston de Carvalho Azevedo3Bertram Brenig4Fernanda Badotti5Aristóteles Góes-Neto6Laboratory of Molecular and Computational Biology of Fungi, Department of Microbiology, Institute of Biological Sciences, Universidade Federal de Minas Gerais (UFMG), Belo Horizonte, 31270-901, MG, Brazil; Graduate Program in Bioinformatics, Institute of Biological Sciences, Universidade Federal de Minas Gerais (UFMG), Belo Horizonte, 31270-901, MG, BrazilLaboratory of Molecular and Computational Biology of Fungi, Department of Microbiology, Institute of Biological Sciences, Universidade Federal de Minas Gerais (UFMG), Belo Horizonte, 31270-901, MG, Brazil; Graduate Program in Bioinformatics, Institute of Biological Sciences, Universidade Federal de Minas Gerais (UFMG), Belo Horizonte, 31270-901, MG, Brazil; Laboratory of Cellular and Molecular Genetics, Institute of Biological Sciences, Universidade Federal de Minas Gerais (UFMG), Belo Horizonte, 31270-901, MG, BrazilLaboratory of Molecular and Computational Biology of Fungi, Department of Microbiology, Institute of Biological Sciences, Universidade Federal de Minas Gerais (UFMG), Belo Horizonte, 31270-901, MG, Brazil; Graduate Program in Bioinformatics, Institute of Biological Sciences, Universidade Federal de Minas Gerais (UFMG), Belo Horizonte, 31270-901, MG, Brazil; Laboratory of Cellular and Molecular Genetics, Institute of Biological Sciences, Universidade Federal de Minas Gerais (UFMG), Belo Horizonte, 31270-901, MG, BrazilGraduate Program in Bioinformatics, Institute of Biological Sciences, Universidade Federal de Minas Gerais (UFMG), Belo Horizonte, 31270-901, MG, Brazil; Laboratory of Cellular and Molecular Genetics, Institute of Biological Sciences, Universidade Federal de Minas Gerais (UFMG), Belo Horizonte, 31270-901, MG, BrazilInstitute of Veterinary Medicine, Burckhardtweg, University of Göttingen, 37073, Göttingen, GermanyDepartment of Chemistry, Centro Federal de Educação Tecnológica de Minas Gerais, Belo Horizonte, 30421-169, MG, BrazilLaboratory of Molecular and Computational Biology of Fungi, Department of Microbiology, Institute of Biological Sciences, Universidade Federal de Minas Gerais (UFMG), Belo Horizonte, 31270-901, MG, Brazil; Graduate Program in Bioinformatics, Institute of Biological Sciences, Universidade Federal de Minas Gerais (UFMG), Belo Horizonte, 31270-901, MG, Brazil; Corresponding author. Laboratory of Molecular and Computational Biology of Fungi, Department of Microbiology, Institute of Biological Sciences, Universidade Federal de Minas Gerais (UFMG), Belo Horizonte, 31270-901, MG, Brazil.Trametes villosa is a remarkable white-rot fungus (WRF) with the potential to be applied in lignocellulose conversion to obtain chemical compounds and biofuels. Lignocellulose breakdown by WRF is carried out through the secretion of oxidative and hydrolytic enzymes. Despite the existing knowledge about this process, the complete molecular mechanisms involved in the regulation of this metabolic system have not yet been elucidated. Therefore, in order to understand the genes and metabolic pathways regulated during lignocellulose degradation, the strain T. villosa CCMB561 was cultured in media with different carbon sources (lignin, sugarcane bagasse, and malt extract). Subsequently, biochemical assays and differential gene expression analysis by qPCR and high-throughput RNA sequencing were carried out. Our results revealed the ability of T. villosa CCMB561 to grow on lignin (AL medium) as the unique carbon source. An overexpression of Cytochrome P450 was detected in this medium, which may be associated with the lignin O-demethylation pathway. Clusters of up-regulated CAZymes-encoding genes were identified in lignin and sugarcane bagasse, revealing that T. villosa CCMB561 acts simultaneously in the depolymerization of lignin, cellulose, hemicellulose, and pectin. Furthermore, genes encoding nitroreductases and homogentisate-1,2-dioxygenase that act in the degradation of organic pollutants were up-regulated in the lignin medium. Altogether, these findings provide new insights into the mechanisms of lignocellulose degradation by T. villosa and confirm the ability of this fungal species to be applied in biorefineries and in the bioremediation of organic pollutants.http://www.sciencedirect.com/science/article/pii/S2405844024044803LignocelluloseCAZymesTranscriptomicsWhite-rot fungiRenewable energySustainability |
spellingShingle | Luiz Marcelo Ribeiro Tomé Mariana Teixeira Dornelles Parise Doglas Parise Vasco Ariston de Carvalho Azevedo Bertram Brenig Fernanda Badotti Aristóteles Góes-Neto Pure lignin induces overexpression of cytochrome P450 (CYP) encoding genes and brings insights into the lignocellulose depolymerization by Trametes villosa Heliyon Lignocellulose CAZymes Transcriptomics White-rot fungi Renewable energy Sustainability |
title | Pure lignin induces overexpression of cytochrome P450 (CYP) encoding genes and brings insights into the lignocellulose depolymerization by Trametes villosa |
title_full | Pure lignin induces overexpression of cytochrome P450 (CYP) encoding genes and brings insights into the lignocellulose depolymerization by Trametes villosa |
title_fullStr | Pure lignin induces overexpression of cytochrome P450 (CYP) encoding genes and brings insights into the lignocellulose depolymerization by Trametes villosa |
title_full_unstemmed | Pure lignin induces overexpression of cytochrome P450 (CYP) encoding genes and brings insights into the lignocellulose depolymerization by Trametes villosa |
title_short | Pure lignin induces overexpression of cytochrome P450 (CYP) encoding genes and brings insights into the lignocellulose depolymerization by Trametes villosa |
title_sort | pure lignin induces overexpression of cytochrome p450 cyp encoding genes and brings insights into the lignocellulose depolymerization by trametes villosa |
topic | Lignocellulose CAZymes Transcriptomics White-rot fungi Renewable energy Sustainability |
url | http://www.sciencedirect.com/science/article/pii/S2405844024044803 |
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