Pure lignin induces overexpression of cytochrome P450 (CYP) encoding genes and brings insights into the lignocellulose depolymerization by Trametes villosa

Trametes villosa is a remarkable white-rot fungus (WRF) with the potential to be applied in lignocellulose conversion to obtain chemical compounds and biofuels. Lignocellulose breakdown by WRF is carried out through the secretion of oxidative and hydrolytic enzymes. Despite the existing knowledge ab...

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Main Authors: Luiz Marcelo Ribeiro Tomé, Mariana Teixeira Dornelles Parise, Doglas Parise, Vasco Ariston de Carvalho Azevedo, Bertram Brenig, Fernanda Badotti, Aristóteles Góes-Neto
Format: Article
Language:English
Published: Elsevier 2024-04-01
Series:Heliyon
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Online Access:http://www.sciencedirect.com/science/article/pii/S2405844024044803
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author Luiz Marcelo Ribeiro Tomé
Mariana Teixeira Dornelles Parise
Doglas Parise
Vasco Ariston de Carvalho Azevedo
Bertram Brenig
Fernanda Badotti
Aristóteles Góes-Neto
author_facet Luiz Marcelo Ribeiro Tomé
Mariana Teixeira Dornelles Parise
Doglas Parise
Vasco Ariston de Carvalho Azevedo
Bertram Brenig
Fernanda Badotti
Aristóteles Góes-Neto
author_sort Luiz Marcelo Ribeiro Tomé
collection DOAJ
description Trametes villosa is a remarkable white-rot fungus (WRF) with the potential to be applied in lignocellulose conversion to obtain chemical compounds and biofuels. Lignocellulose breakdown by WRF is carried out through the secretion of oxidative and hydrolytic enzymes. Despite the existing knowledge about this process, the complete molecular mechanisms involved in the regulation of this metabolic system have not yet been elucidated. Therefore, in order to understand the genes and metabolic pathways regulated during lignocellulose degradation, the strain T. villosa CCMB561 was cultured in media with different carbon sources (lignin, sugarcane bagasse, and malt extract). Subsequently, biochemical assays and differential gene expression analysis by qPCR and high-throughput RNA sequencing were carried out. Our results revealed the ability of T. villosa CCMB561 to grow on lignin (AL medium) as the unique carbon source. An overexpression of Cytochrome P450 was detected in this medium, which may be associated with the lignin O-demethylation pathway. Clusters of up-regulated CAZymes-encoding genes were identified in lignin and sugarcane bagasse, revealing that T. villosa CCMB561 acts simultaneously in the depolymerization of lignin, cellulose, hemicellulose, and pectin. Furthermore, genes encoding nitroreductases and homogentisate-1,2-dioxygenase that act in the degradation of organic pollutants were up-regulated in the lignin medium. Altogether, these findings provide new insights into the mechanisms of lignocellulose degradation by T. villosa and confirm the ability of this fungal species to be applied in biorefineries and in the bioremediation of organic pollutants.
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spelling doaj.art-f01ce11b357c40d285bfea09ad55c5a32024-03-28T06:38:26ZengElsevierHeliyon2405-84402024-04-01107e28449Pure lignin induces overexpression of cytochrome P450 (CYP) encoding genes and brings insights into the lignocellulose depolymerization by Trametes villosaLuiz Marcelo Ribeiro Tomé0Mariana Teixeira Dornelles Parise1Doglas Parise2Vasco Ariston de Carvalho Azevedo3Bertram Brenig4Fernanda Badotti5Aristóteles Góes-Neto6Laboratory of Molecular and Computational Biology of Fungi, Department of Microbiology, Institute of Biological Sciences, Universidade Federal de Minas Gerais (UFMG), Belo Horizonte, 31270-901, MG, Brazil; Graduate Program in Bioinformatics, Institute of Biological Sciences, Universidade Federal de Minas Gerais (UFMG), Belo Horizonte, 31270-901, MG, BrazilLaboratory of Molecular and Computational Biology of Fungi, Department of Microbiology, Institute of Biological Sciences, Universidade Federal de Minas Gerais (UFMG), Belo Horizonte, 31270-901, MG, Brazil; Graduate Program in Bioinformatics, Institute of Biological Sciences, Universidade Federal de Minas Gerais (UFMG), Belo Horizonte, 31270-901, MG, Brazil; Laboratory of Cellular and Molecular Genetics, Institute of Biological Sciences, Universidade Federal de Minas Gerais (UFMG), Belo Horizonte, 31270-901, MG, BrazilLaboratory of Molecular and Computational Biology of Fungi, Department of Microbiology, Institute of Biological Sciences, Universidade Federal de Minas Gerais (UFMG), Belo Horizonte, 31270-901, MG, Brazil; Graduate Program in Bioinformatics, Institute of Biological Sciences, Universidade Federal de Minas Gerais (UFMG), Belo Horizonte, 31270-901, MG, Brazil; Laboratory of Cellular and Molecular Genetics, Institute of Biological Sciences, Universidade Federal de Minas Gerais (UFMG), Belo Horizonte, 31270-901, MG, BrazilGraduate Program in Bioinformatics, Institute of Biological Sciences, Universidade Federal de Minas Gerais (UFMG), Belo Horizonte, 31270-901, MG, Brazil; Laboratory of Cellular and Molecular Genetics, Institute of Biological Sciences, Universidade Federal de Minas Gerais (UFMG), Belo Horizonte, 31270-901, MG, BrazilInstitute of Veterinary Medicine, Burckhardtweg, University of Göttingen, 37073, Göttingen, GermanyDepartment of Chemistry, Centro Federal de Educação Tecnológica de Minas Gerais, Belo Horizonte, 30421-169, MG, BrazilLaboratory of Molecular and Computational Biology of Fungi, Department of Microbiology, Institute of Biological Sciences, Universidade Federal de Minas Gerais (UFMG), Belo Horizonte, 31270-901, MG, Brazil; Graduate Program in Bioinformatics, Institute of Biological Sciences, Universidade Federal de Minas Gerais (UFMG), Belo Horizonte, 31270-901, MG, Brazil; Corresponding author. Laboratory of Molecular and Computational Biology of Fungi, Department of Microbiology, Institute of Biological Sciences, Universidade Federal de Minas Gerais (UFMG), Belo Horizonte, 31270-901, MG, Brazil.Trametes villosa is a remarkable white-rot fungus (WRF) with the potential to be applied in lignocellulose conversion to obtain chemical compounds and biofuels. Lignocellulose breakdown by WRF is carried out through the secretion of oxidative and hydrolytic enzymes. Despite the existing knowledge about this process, the complete molecular mechanisms involved in the regulation of this metabolic system have not yet been elucidated. Therefore, in order to understand the genes and metabolic pathways regulated during lignocellulose degradation, the strain T. villosa CCMB561 was cultured in media with different carbon sources (lignin, sugarcane bagasse, and malt extract). Subsequently, biochemical assays and differential gene expression analysis by qPCR and high-throughput RNA sequencing were carried out. Our results revealed the ability of T. villosa CCMB561 to grow on lignin (AL medium) as the unique carbon source. An overexpression of Cytochrome P450 was detected in this medium, which may be associated with the lignin O-demethylation pathway. Clusters of up-regulated CAZymes-encoding genes were identified in lignin and sugarcane bagasse, revealing that T. villosa CCMB561 acts simultaneously in the depolymerization of lignin, cellulose, hemicellulose, and pectin. Furthermore, genes encoding nitroreductases and homogentisate-1,2-dioxygenase that act in the degradation of organic pollutants were up-regulated in the lignin medium. Altogether, these findings provide new insights into the mechanisms of lignocellulose degradation by T. villosa and confirm the ability of this fungal species to be applied in biorefineries and in the bioremediation of organic pollutants.http://www.sciencedirect.com/science/article/pii/S2405844024044803LignocelluloseCAZymesTranscriptomicsWhite-rot fungiRenewable energySustainability
spellingShingle Luiz Marcelo Ribeiro Tomé
Mariana Teixeira Dornelles Parise
Doglas Parise
Vasco Ariston de Carvalho Azevedo
Bertram Brenig
Fernanda Badotti
Aristóteles Góes-Neto
Pure lignin induces overexpression of cytochrome P450 (CYP) encoding genes and brings insights into the lignocellulose depolymerization by Trametes villosa
Heliyon
Lignocellulose
CAZymes
Transcriptomics
White-rot fungi
Renewable energy
Sustainability
title Pure lignin induces overexpression of cytochrome P450 (CYP) encoding genes and brings insights into the lignocellulose depolymerization by Trametes villosa
title_full Pure lignin induces overexpression of cytochrome P450 (CYP) encoding genes and brings insights into the lignocellulose depolymerization by Trametes villosa
title_fullStr Pure lignin induces overexpression of cytochrome P450 (CYP) encoding genes and brings insights into the lignocellulose depolymerization by Trametes villosa
title_full_unstemmed Pure lignin induces overexpression of cytochrome P450 (CYP) encoding genes and brings insights into the lignocellulose depolymerization by Trametes villosa
title_short Pure lignin induces overexpression of cytochrome P450 (CYP) encoding genes and brings insights into the lignocellulose depolymerization by Trametes villosa
title_sort pure lignin induces overexpression of cytochrome p450 cyp encoding genes and brings insights into the lignocellulose depolymerization by trametes villosa
topic Lignocellulose
CAZymes
Transcriptomics
White-rot fungi
Renewable energy
Sustainability
url http://www.sciencedirect.com/science/article/pii/S2405844024044803
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