Generation of Oxygen Free Radicals by Proflavine: Implication in Protein Degradation
Proflavine, an acridine dye, is a known DNA intercalating agent. In the present study, we show that proflavine alone on photoillumination can generate reactive oxygen species (ROS). These proflavine-derived ROS cause damage to proteins, and this effect is enhanced when the divalent metal ion Cu (II)...
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| Format: | Article |
| Language: | English |
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Bogor Agricultural University
2018-03-01
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| Series: | Hayati Journal of Biosciences |
| Subjects: | |
| Online Access: | https://journal.ipb.ac.id/index.php/hayati/article/view/20802 |
| _version_ | 1811159017404235776 |
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| author | Mansour K.M. Gatasheh Kannan Subbaram Hemalatha Kannan Imrana Naseem |
| author_facet | Mansour K.M. Gatasheh Kannan Subbaram Hemalatha Kannan Imrana Naseem |
| author_sort | Mansour K.M. Gatasheh |
| collection | DOAJ |
| description | Proflavine, an acridine dye, is a known DNA intercalating agent. In the present study, we show that proflavine alone on photoillumination can generate reactive oxygen species (ROS). These proflavine-derived ROS cause damage to proteins, and this effect is enhanced when the divalent metal ion Cu (II) is included in the reaction. Bathocuproine, a specific Cu (I) sequestering agent, when present in the reaction mixture containing Cu (II), was found to inhibit the protein degradation, showing that Cu (I) is an essential intermediate in the reaction. The effect of several scavengers of ROS such as superoxide dismutase, sodium azide, potassium iodide, and thiourea were examined on the protein damaging reaction. Potassium iodide was found to be the most effective in inhibiting protein damage followed by sodium azide and thiourea. Our results indicate the involvement of superoxide, singlet oxygen, triplet oxygen, and hydroxyl radicals in proflavine-induced damage to proteins. |
| first_indexed | 2024-04-10T05:34:02Z |
| format | Article |
| id | doaj.art-f02ce3a500864c70bba09c137dba9b44 |
| institution | Directory Open Access Journal |
| issn | 1978-3019 2086-4094 |
| language | English |
| last_indexed | 2024-04-10T05:34:02Z |
| publishDate | 2018-03-01 |
| publisher | Bogor Agricultural University |
| record_format | Article |
| series | Hayati Journal of Biosciences |
| spelling | doaj.art-f02ce3a500864c70bba09c137dba9b442023-03-07T02:22:17ZengBogor Agricultural UniversityHayati Journal of Biosciences1978-30192086-40942018-03-0124310.4308/hjb.24.3.118Generation of Oxygen Free Radicals by Proflavine: Implication in Protein DegradationMansour K.M. Gatasheh0Kannan Subbaram1Hemalatha Kannan2Imrana Naseem3Department of Clinical Laboratory Sciences, Al-Ghad International Colleges for Applied Medical Sciences, Ar-Rafiah, RiyadhDepartment of Preparatory, Al-Ghad International Colleges for Applied Medical Sciences, Ar-Rafiah, RiyadhDepartment of Laboratory Sciences & Pathology, Jimma UniversityDepartment of Biochemistry, Faculty of Life Sciences, Aligarh Muslim University, AligarhProflavine, an acridine dye, is a known DNA intercalating agent. In the present study, we show that proflavine alone on photoillumination can generate reactive oxygen species (ROS). These proflavine-derived ROS cause damage to proteins, and this effect is enhanced when the divalent metal ion Cu (II) is included in the reaction. Bathocuproine, a specific Cu (I) sequestering agent, when present in the reaction mixture containing Cu (II), was found to inhibit the protein degradation, showing that Cu (I) is an essential intermediate in the reaction. The effect of several scavengers of ROS such as superoxide dismutase, sodium azide, potassium iodide, and thiourea were examined on the protein damaging reaction. Potassium iodide was found to be the most effective in inhibiting protein damage followed by sodium azide and thiourea. Our results indicate the involvement of superoxide, singlet oxygen, triplet oxygen, and hydroxyl radicals in proflavine-induced damage to proteins.https://journal.ipb.ac.id/index.php/hayati/article/view/20802bovine serum albuminphotoilluminationproflavineprotein degradationreactive oxygen species |
| spellingShingle | Mansour K.M. Gatasheh Kannan Subbaram Hemalatha Kannan Imrana Naseem Generation of Oxygen Free Radicals by Proflavine: Implication in Protein Degradation Hayati Journal of Biosciences bovine serum albumin photoillumination proflavine protein degradation reactive oxygen species |
| title | Generation of Oxygen Free Radicals by Proflavine: Implication in Protein Degradation |
| title_full | Generation of Oxygen Free Radicals by Proflavine: Implication in Protein Degradation |
| title_fullStr | Generation of Oxygen Free Radicals by Proflavine: Implication in Protein Degradation |
| title_full_unstemmed | Generation of Oxygen Free Radicals by Proflavine: Implication in Protein Degradation |
| title_short | Generation of Oxygen Free Radicals by Proflavine: Implication in Protein Degradation |
| title_sort | generation of oxygen free radicals by proflavine implication in protein degradation |
| topic | bovine serum albumin photoillumination proflavine protein degradation reactive oxygen species |
| url | https://journal.ipb.ac.id/index.php/hayati/article/view/20802 |
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