A Novel High-Throughput Assay Reveals That the Temperature Induced Increases in Transphosphatidylation of Phospholipase D Are Dependent on the Alcohol Acceptor Concentration

Phospholipase D reacts with alcohols or water, transphosphatidylating or hydrolysing lipids such as phosphatidylcholine, generating phosphatidylalcohols or phosphatidic acid, respectively. The enzyme has been employed in many applications making use of the transphosphatidylation reaction and the enz...

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Bibliographic Details
Main Authors: Hengzhang Yang, Rüdiger Woscholski
Format: Article
Language:English
Published: MDPI AG 2022-04-01
Series:Biomolecules
Subjects:
Online Access:https://www.mdpi.com/2218-273X/12/5/632