sgRNA design and in vitro nucleolytic analysis of the Cas9‐RNP complex for transgene‐free genome editing of the eIF4E1 gene from Capsicum an‐ nuum L.

sgRNA design and in vitro nucleolytic analysis of the Cas9‐RNP complex for transgene‐free genome editing of the eIF4E1 gene from Capsicum an‐ nuum L.

Chili (Capsicum annuum L.) is a highly valued vegetable, renowned for its unique taste and aroma. However, chili production faces challenges in meeting the high demand due to infections caused by pathogens such as ChiVMV (potyvirus). Previous studies have suggested that chili eIF4E1 plays a crucial...

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Bibliographic Details
Main Authors: Josefanny Tham, Alfred Patisenah, Tommy Octavianus Soetrisno Tjia, Santiago Signorelli, Intan Taufik, Karlia Meitha
Format: Article
Language:English
Published: Universitas Gadjah Mada, Yogyakarta 2023-12-01
Series:Indonesian Journal of Biotechnology
Subjects:
crispr‐cas9
endonuclease
recombinant protein
sgrna cas9‐rnp
Online Access:https://jurnal.ugm.ac.id/ijbiotech/article/view/86778

Internet

https://jurnal.ugm.ac.id/ijbiotech/article/view/86778

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