Dimethyl sulfoxide improves sensitivity and specificity of RT-PCR and qRT-PCR amplification of low-expressed transgenes

Dimethyl sulfoxide improves sensitivity and specificity of RT-PCR and qRT-PCR amplification of low-expressed transgenes

The expression of transgenes in a host plant may be low for a number of reasons. Both low expression and poor specificity of amplification were encountered during analysis of the expression of the Arabidopsis cytokinin oxidase/dehydrogenase (AtCKX1) gene in transgenic Centaurium erythraea. The o...

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Bibliographic Details
Main Authors: Trifunović Milana, Simonović Ana, Bogdanović Milica, Cingel A., Raspor M., Subotić Angelina, Dragićević M.
Format: Article
Language:English
Published: University of Belgrade, University of Novi Sad 2012-01-01
Series:Archives of Biological Sciences
Subjects:
Bovine serum albumin
dimethyl sulfoxide
ethylene glycol
formamide
glycerol
PCR additives
PCR enhancers
qPCR optimization
trehalose
Tween-20
Online Access:http://www.doiserbia.nb.rs/img/doi/0354-4664/2012/0354-46641203865S.pdf

Internet

http://www.doiserbia.nb.rs/img/doi/0354-4664/2012/0354-46641203865S.pdf

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