Evaluation of heterologous expression in Pichia pastoris of Pine Weevil TRPA1 by GFP and flow cytometry

Evaluation of heterologous expression in Pichia pastoris of Pine Weevil TRPA1 by GFP and flow cytometry

Abstract Background The wasabi receptor, also known as the Transient Receptor Potential Ankyrin 1 (TRPA1) ion channel, is a potential target for development of repellents for insects, like the pine weevil (Hylobius abietis) feeding on conifer seedlings and causing damage in forestry. Heterologous ex...

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Bibliographic Details
Main Authors: Balder Werin, Wilhelm Hansson Wennersten, Robin Olsson, Oliwia Kołodziejczyk, Martin N. Andersson, Magnus Carlquist, Urban Johanson
Format: Article
Language:English
Published: BMC 2024-04-01
Series:Microbial Cell Factories
Subjects:
Flow cytometry
Green Fluorescent Protein
Hylobius abietis
TRPA1
Membrane protein
Viability
Online Access:https://doi.org/10.1186/s12934-024-02382-5

Internet

https://doi.org/10.1186/s12934-024-02382-5

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