Antibody Response to Influenza Hemagglutinin Conserved Stalk Domain after Sequential Immunization with Old Vaccine Strains
Hemagglutinin (HA) is the major envelope glycoprotein and antigen on the surface of influenza virions. The glycoprotein comprises a globular head and a stalk region. While immunodominant epitopes on influenza HA head are highly variable, the stalk domain is conserved. The variability of the HA head...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Hindawi Limited
2024-01-01
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| Series: | Advances in Virology |
| Online Access: | http://dx.doi.org/10.1155/2024/5691673 |
| _version_ | 1797301899374886912 |
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| author | Alita Kongchanagul Promsin Masrinoul Chompunuch Boonarkart Ornpreya Suptawiwat Prasert Auewarakul |
| author_facet | Alita Kongchanagul Promsin Masrinoul Chompunuch Boonarkart Ornpreya Suptawiwat Prasert Auewarakul |
| author_sort | Alita Kongchanagul |
| collection | DOAJ |
| description | Hemagglutinin (HA) is the major envelope glycoprotein and antigen on the surface of influenza virions. The glycoprotein comprises a globular head and a stalk region. While immunodominant epitopes on influenza HA head are highly variable, the stalk domain is conserved. The variability of the HA head causes the antigenic drift that made the requirement of annual update of vaccine strains. Induction of antibody against the stalk domain has been proposed as an approach for a broadly protective influenza vaccine strategy. Sequential exposure to influenza strains with highly diverse HA heads but conserved stalks have been shown to induce antibody to the low immunogenic stalk domain. Here, we tested this approach by using old influenza vaccine strains that are decades apart in evolution. Inactivated whole virion vaccine of influenza A/Puerto Rico/8/1934, A/USSR/92/1977, and A/Thailand/102/2009 (H1N1) was sequentially immunized into BALB/c mice in comparison to immunization using single strain (A/Thailand/102/2009 (H1N1)). The sequentially immunized mice developed higher levels of binding antibody to the stalk domain. These suggested that using old vaccine strains in sequential vaccination may be a possible approach to induce antibody to the conserved stalk domain. |
| first_indexed | 2024-03-07T23:29:09Z |
| format | Article |
| id | doaj.art-f09527076976437b92fe00bcadeab5cc |
| institution | Directory Open Access Journal |
| issn | 1687-8647 |
| language | English |
| last_indexed | 2024-03-07T23:29:09Z |
| publishDate | 2024-01-01 |
| publisher | Hindawi Limited |
| record_format | Article |
| series | Advances in Virology |
| spelling | doaj.art-f09527076976437b92fe00bcadeab5cc2024-02-21T00:00:02ZengHindawi LimitedAdvances in Virology1687-86472024-01-01202410.1155/2024/5691673Antibody Response to Influenza Hemagglutinin Conserved Stalk Domain after Sequential Immunization with Old Vaccine StrainsAlita Kongchanagul0Promsin Masrinoul1Chompunuch Boonarkart2Ornpreya Suptawiwat3Prasert Auewarakul4Center for Vaccine DevelopmentCenter for Vaccine DevelopmentDepartment of MicrobiologyCenter of Learning and Research in Celebration of HRH Princess Chulabhorn’s 60th Birthday AnniversaryDepartment of MicrobiologyHemagglutinin (HA) is the major envelope glycoprotein and antigen on the surface of influenza virions. The glycoprotein comprises a globular head and a stalk region. While immunodominant epitopes on influenza HA head are highly variable, the stalk domain is conserved. The variability of the HA head causes the antigenic drift that made the requirement of annual update of vaccine strains. Induction of antibody against the stalk domain has been proposed as an approach for a broadly protective influenza vaccine strategy. Sequential exposure to influenza strains with highly diverse HA heads but conserved stalks have been shown to induce antibody to the low immunogenic stalk domain. Here, we tested this approach by using old influenza vaccine strains that are decades apart in evolution. Inactivated whole virion vaccine of influenza A/Puerto Rico/8/1934, A/USSR/92/1977, and A/Thailand/102/2009 (H1N1) was sequentially immunized into BALB/c mice in comparison to immunization using single strain (A/Thailand/102/2009 (H1N1)). The sequentially immunized mice developed higher levels of binding antibody to the stalk domain. These suggested that using old vaccine strains in sequential vaccination may be a possible approach to induce antibody to the conserved stalk domain.http://dx.doi.org/10.1155/2024/5691673 |
| spellingShingle | Alita Kongchanagul Promsin Masrinoul Chompunuch Boonarkart Ornpreya Suptawiwat Prasert Auewarakul Antibody Response to Influenza Hemagglutinin Conserved Stalk Domain after Sequential Immunization with Old Vaccine Strains Advances in Virology |
| title | Antibody Response to Influenza Hemagglutinin Conserved Stalk Domain after Sequential Immunization with Old Vaccine Strains |
| title_full | Antibody Response to Influenza Hemagglutinin Conserved Stalk Domain after Sequential Immunization with Old Vaccine Strains |
| title_fullStr | Antibody Response to Influenza Hemagglutinin Conserved Stalk Domain after Sequential Immunization with Old Vaccine Strains |
| title_full_unstemmed | Antibody Response to Influenza Hemagglutinin Conserved Stalk Domain after Sequential Immunization with Old Vaccine Strains |
| title_short | Antibody Response to Influenza Hemagglutinin Conserved Stalk Domain after Sequential Immunization with Old Vaccine Strains |
| title_sort | antibody response to influenza hemagglutinin conserved stalk domain after sequential immunization with old vaccine strains |
| url | http://dx.doi.org/10.1155/2024/5691673 |
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