Change of Amino Acid Residues in Idiotypic Nanobodies Enhanced the Sensitivity of Competitive Enzyme Immunoassay for Mycotoxin Ochratoxin A in Cereals

Anti-idiotypic nanobodies, usually expressed by gene engineering protocol, has been shown as a nontoxic coating antigen for toxic compound immunoassays. We here focused on how to increase immunoassay sensitivity by changing the nanobody’s primary sequence. In the experiments, two anti-idiotype nanob...

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Bibliographic Details
Main Authors: Caixia Zhang, Weiqi Zhang, Xiaoqian Tang, Qi Zhang, Wen Zhang, Peiwu Li
Format: Article
Language:English
Published: MDPI AG 2020-04-01
Series:Toxins
Subjects:
Online Access:https://www.mdpi.com/2072-6651/12/4/273