Xylitol Production from Pineapple Cores (<i>Ananas comosus</i> (L.) <i>Merr</i>) by Enzymatic and Acid Hydrolysis Using Microorganisms <i>Debaryomyces hansenii</i> and <i>Candida tropicalis</i>
Hydrolysis and fermentation processes are key stages in xylitol production from lignocellulosic materials. In this study, pineapple cores, one of the wastes from the canned pineapple industry, were used as raw material for xylitol production. Two methods was used for hydrolysis: enzymatically using...
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MDPI AG
2022-11-01
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| Online Access: | https://www.mdpi.com/2311-5637/8/12/694 |
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| author | Efri Mardawati Agus T. Hartono Bambang Nurhadi Hana Nur Fitriana Euis Hermiati Riksfardini Annisa Ermawar |
| author_facet | Efri Mardawati Agus T. Hartono Bambang Nurhadi Hana Nur Fitriana Euis Hermiati Riksfardini Annisa Ermawar |
| author_sort | Efri Mardawati |
| collection | DOAJ |
| description | Hydrolysis and fermentation processes are key stages in xylitol production from lignocellulosic materials. In this study, pineapple cores, one of the wastes from the canned pineapple industry, were used as raw material for xylitol production. Two methods was used for hydrolysis: enzymatically using commercial enzyme Cellic HTec2, and acid hydrolysis using 4% H<sub>2</sub>SO<sub>4</sub>. In contrast, the fermentation process was carried out with two selected yeasts commonly employed in xylitol fermentation, <i>Debaryomycess hansenii</i>, and <i>Candida tropicalis</i>. Before these two processes, the pineapple cores were characterized using the Van Soest method to determine their lignocellulosic content. The hemicellulose content was 36.06%, the cellulose content was 14.20%, and the lignin content was 10.05%. This result indicates that the hemicellulose content of pineapple cores has the potential to be used as a raw material in the production of xylitol. The hydrolysis efficiency of enzymatic hydrolysis was 21% higher than that of acid hydrolysis. The highest xylitol and biomass yield of 0.371 g<sub>xylitol</sub>/g<sub>xylose</sub> and 0.225 g<sub>cell</sub>/g<sub>xylose</sub> were observed by <i>C. tropicalis</i> using an enzymatic hydrolysate. |
| first_indexed | 2024-03-09T16:47:27Z |
| format | Article |
| id | doaj.art-f0fa3d0403024380add3fccc8ce4f514 |
| institution | Directory Open Access Journal |
| issn | 2311-5637 |
| language | English |
| last_indexed | 2024-03-09T16:47:27Z |
| publishDate | 2022-11-01 |
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| series | Fermentation |
| spelling | doaj.art-f0fa3d0403024380add3fccc8ce4f5142023-11-24T14:45:11ZengMDPI AGFermentation2311-56372022-11-0181269410.3390/fermentation8120694Xylitol Production from Pineapple Cores (<i>Ananas comosus</i> (L.) <i>Merr</i>) by Enzymatic and Acid Hydrolysis Using Microorganisms <i>Debaryomyces hansenii</i> and <i>Candida tropicalis</i>Efri Mardawati0Agus T. Hartono1Bambang Nurhadi2Hana Nur Fitriana3Euis Hermiati4Riksfardini Annisa Ermawar5Department of Agroindustrial Technology, Universitas Padjadjaran, Jatinangor 45363, IndonesiaDepartment of Agroindustrial Technology, Universitas Padjadjaran, Jatinangor 45363, IndonesiaResearch Collaboration Center for Biomass and Biorefinery between BRIN and Universitas Padjadjaran, Jatinangor 45363, IndonesiaDepartment of Agroindustrial Technology, Universitas Padjadjaran, Jatinangor 45363, IndonesiaResearch Collaboration Center for Biomass and Biorefinery between BRIN and Universitas Padjadjaran, Jatinangor 45363, IndonesiaResearch Collaboration Center for Biomass and Biorefinery between BRIN and Universitas Padjadjaran, Jatinangor 45363, IndonesiaHydrolysis and fermentation processes are key stages in xylitol production from lignocellulosic materials. In this study, pineapple cores, one of the wastes from the canned pineapple industry, were used as raw material for xylitol production. Two methods was used for hydrolysis: enzymatically using commercial enzyme Cellic HTec2, and acid hydrolysis using 4% H<sub>2</sub>SO<sub>4</sub>. In contrast, the fermentation process was carried out with two selected yeasts commonly employed in xylitol fermentation, <i>Debaryomycess hansenii</i>, and <i>Candida tropicalis</i>. Before these two processes, the pineapple cores were characterized using the Van Soest method to determine their lignocellulosic content. The hemicellulose content was 36.06%, the cellulose content was 14.20%, and the lignin content was 10.05%. This result indicates that the hemicellulose content of pineapple cores has the potential to be used as a raw material in the production of xylitol. The hydrolysis efficiency of enzymatic hydrolysis was 21% higher than that of acid hydrolysis. The highest xylitol and biomass yield of 0.371 g<sub>xylitol</sub>/g<sub>xylose</sub> and 0.225 g<sub>cell</sub>/g<sub>xylose</sub> were observed by <i>C. tropicalis</i> using an enzymatic hydrolysate.https://www.mdpi.com/2311-5637/8/12/694pineapple cores<i>Candida tropicalis</i><i>Debaryomycess hansenii</i>enzymatic hydrolysisacid hydrolysisxylitol |
| spellingShingle | Efri Mardawati Agus T. Hartono Bambang Nurhadi Hana Nur Fitriana Euis Hermiati Riksfardini Annisa Ermawar Xylitol Production from Pineapple Cores (<i>Ananas comosus</i> (L.) <i>Merr</i>) by Enzymatic and Acid Hydrolysis Using Microorganisms <i>Debaryomyces hansenii</i> and <i>Candida tropicalis</i> Fermentation pineapple cores <i>Candida tropicalis</i> <i>Debaryomycess hansenii</i> enzymatic hydrolysis acid hydrolysis xylitol |
| title | Xylitol Production from Pineapple Cores (<i>Ananas comosus</i> (L.) <i>Merr</i>) by Enzymatic and Acid Hydrolysis Using Microorganisms <i>Debaryomyces hansenii</i> and <i>Candida tropicalis</i> |
| title_full | Xylitol Production from Pineapple Cores (<i>Ananas comosus</i> (L.) <i>Merr</i>) by Enzymatic and Acid Hydrolysis Using Microorganisms <i>Debaryomyces hansenii</i> and <i>Candida tropicalis</i> |
| title_fullStr | Xylitol Production from Pineapple Cores (<i>Ananas comosus</i> (L.) <i>Merr</i>) by Enzymatic and Acid Hydrolysis Using Microorganisms <i>Debaryomyces hansenii</i> and <i>Candida tropicalis</i> |
| title_full_unstemmed | Xylitol Production from Pineapple Cores (<i>Ananas comosus</i> (L.) <i>Merr</i>) by Enzymatic and Acid Hydrolysis Using Microorganisms <i>Debaryomyces hansenii</i> and <i>Candida tropicalis</i> |
| title_short | Xylitol Production from Pineapple Cores (<i>Ananas comosus</i> (L.) <i>Merr</i>) by Enzymatic and Acid Hydrolysis Using Microorganisms <i>Debaryomyces hansenii</i> and <i>Candida tropicalis</i> |
| title_sort | xylitol production from pineapple cores i ananas comosus i l i merr i by enzymatic and acid hydrolysis using microorganisms i debaryomyces hansenii i and i candida tropicalis i |
| topic | pineapple cores <i>Candida tropicalis</i> <i>Debaryomycess hansenii</i> enzymatic hydrolysis acid hydrolysis xylitol |
| url | https://www.mdpi.com/2311-5637/8/12/694 |
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