MICROBIOLOGICAL CONFIRMATION OF A FOCUS OF ACTIVE TRACHOMA IN AMERINDIANS OF COLOMBIA, SOUTH AMERICA

Objective. To confirm the microbiological presence of C. trachomatis in indigenous communities of Colombia found to have cases of active trachoma. Method: An ophthalmic evaluation was performed in order to identify the active forms of the disease. Ocular swabs were taken from the conjunctiva and an...

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Main Authors: Fernando Yaacov Peña, Elkin Hernandez, Hollman Miller, Adriana Gomez, Viviana Ramos, Jaime Moreno
Format: Article
Language:English
Published: Pan-American Association of Ophthalmology 2016-02-01
Series:Vision Pan-America
Subjects:
Online Access:http://journals.sfu.ca/paao/index.php/journal/article/view/307
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author Fernando Yaacov Peña
Elkin Hernandez
Hollman Miller
Adriana Gomez
Viviana Ramos
Jaime Moreno
author_facet Fernando Yaacov Peña
Elkin Hernandez
Hollman Miller
Adriana Gomez
Viviana Ramos
Jaime Moreno
author_sort Fernando Yaacov Peña
collection DOAJ
description Objective. To confirm the microbiological presence of C. trachomatis in indigenous communities of Colombia found to have cases of active trachoma. Method: An ophthalmic evaluation was performed in order to identify the active forms of the disease. Ocular swabs were taken from the conjunctiva and analyzed by real time PCR “in house” for the detection of C. trachomatis. Positive samples, were confirmed by a commercial real time PCR and DNA sequencing. Design: Descriptive study Subjects: Children with active trachoma from different indigenous communities in Vaupés, Colombia. Main outcome measures: Presence of C. trachomatis in conjunctival swab samples Results. Fifteen clinical cases of active trachoma were identified in children between one and 12 years old. A major proportion of TI was observed, compared with TF and greater bilateral commitment of the disease. Patients were from the indigenous communities of Santa Catalina (73.3%), Pacuativa (20%) and Piramirí (6.7%). The presence of C. trachomatis was confirmed in 3 (20%) patients, by real time PCR technique “in house” and confirmatory, and validated with sequencing. Conclusion. This study confirms the presence of C. trachomatis in cases of active trachoma in indigenous communities of the department of Vaupés, Colombia.
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spelling doaj.art-f1094c59eca348949199064fde4424002022-12-22T01:32:37ZengPan-American Association of OphthalmologyVision Pan-America2219-46652219-46732016-02-01152434710.15324/vpa.v15i2.307222MICROBIOLOGICAL CONFIRMATION OF A FOCUS OF ACTIVE TRACHOMA IN AMERINDIANS OF COLOMBIA, SOUTH AMERICAFernando Yaacov Peña0Elkin Hernandez1Hollman Miller2Adriana Gomez3Viviana Ramos4Jaime Moreno5Ophthalmologist, Research Unit. Universidad El Bosque, SightFirst, OjoSaludPhysician, Microbiologist. Instituto Nacional de Salud. Bogota ColombiaVector-transmited Disease Program, Vaupes Health State Secretary, Mitú, Vaupés, Colombia,Microbiologist. Microbiology Group, National Health Institute, Bogotá, ColombiaMicrobiology Group, National Health Institute, Bogotá, ColombiaMicrobiology Group, National Health Institute, Bogotá, ColombiaObjective. To confirm the microbiological presence of C. trachomatis in indigenous communities of Colombia found to have cases of active trachoma. Method: An ophthalmic evaluation was performed in order to identify the active forms of the disease. Ocular swabs were taken from the conjunctiva and analyzed by real time PCR “in house” for the detection of C. trachomatis. Positive samples, were confirmed by a commercial real time PCR and DNA sequencing. Design: Descriptive study Subjects: Children with active trachoma from different indigenous communities in Vaupés, Colombia. Main outcome measures: Presence of C. trachomatis in conjunctival swab samples Results. Fifteen clinical cases of active trachoma were identified in children between one and 12 years old. A major proportion of TI was observed, compared with TF and greater bilateral commitment of the disease. Patients were from the indigenous communities of Santa Catalina (73.3%), Pacuativa (20%) and Piramirí (6.7%). The presence of C. trachomatis was confirmed in 3 (20%) patients, by real time PCR technique “in house” and confirmatory, and validated with sequencing. Conclusion. This study confirms the presence of C. trachomatis in cases of active trachoma in indigenous communities of the department of Vaupés, Colombia.http://journals.sfu.ca/paao/index.php/journal/article/view/307Chlamydia trachomatis, trachoma, indigenous, real time PCR, blindness, primary eye care
spellingShingle Fernando Yaacov Peña
Elkin Hernandez
Hollman Miller
Adriana Gomez
Viviana Ramos
Jaime Moreno
MICROBIOLOGICAL CONFIRMATION OF A FOCUS OF ACTIVE TRACHOMA IN AMERINDIANS OF COLOMBIA, SOUTH AMERICA
Vision Pan-America
Chlamydia trachomatis, trachoma, indigenous, real time PCR, blindness, primary eye care
title MICROBIOLOGICAL CONFIRMATION OF A FOCUS OF ACTIVE TRACHOMA IN AMERINDIANS OF COLOMBIA, SOUTH AMERICA
title_full MICROBIOLOGICAL CONFIRMATION OF A FOCUS OF ACTIVE TRACHOMA IN AMERINDIANS OF COLOMBIA, SOUTH AMERICA
title_fullStr MICROBIOLOGICAL CONFIRMATION OF A FOCUS OF ACTIVE TRACHOMA IN AMERINDIANS OF COLOMBIA, SOUTH AMERICA
title_full_unstemmed MICROBIOLOGICAL CONFIRMATION OF A FOCUS OF ACTIVE TRACHOMA IN AMERINDIANS OF COLOMBIA, SOUTH AMERICA
title_short MICROBIOLOGICAL CONFIRMATION OF A FOCUS OF ACTIVE TRACHOMA IN AMERINDIANS OF COLOMBIA, SOUTH AMERICA
title_sort microbiological confirmation of a focus of active trachoma in amerindians of colombia south america
topic Chlamydia trachomatis, trachoma, indigenous, real time PCR, blindness, primary eye care
url http://journals.sfu.ca/paao/index.php/journal/article/view/307
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