Tag-Free SARS-CoV-2 Receptor Binding Domain (RBD), but Not C-Terminal Tagged SARS-CoV-2 RBD, Induces a Rapid and Potent Neutralizing Antibody Response

Tag-Free SARS-CoV-2 Receptor Binding Domain (RBD), but Not C-Terminal Tagged SARS-CoV-2 RBD, Induces a Rapid and Potent Neutralizing Antibody Response

Recombinant proteins are essential in the development of subunit vaccines. In the design of many recombinant proteins, polyhistidine residues are added to the N- or C-termini of target sequences to facilitate purification. However, whether the addition of tag residues influences the immunogenicity o...

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Bibliographic Details
Main Authors: Ting-Wei Lin, Ping-Han Huang, Bo-Hung Liao, Tai-Ling Chao, Ya-Min Tsai, Shih-Chung Chang, Sui-Yuan Chang, Hui-Wen Chen
Format: Article
Language:English
Published: MDPI AG 2022-10-01
Series:Vaccines
Subjects:
COVID-19
vaccine
tag-free RBD
His-tag RBD
neutralizing antibodies
Delta variant
Online Access:https://www.mdpi.com/2076-393X/10/11/1839

Internet

https://www.mdpi.com/2076-393X/10/11/1839

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