A randomized multiplex CRISPRi-Seq approach for the identification of critical combinations of genes

A randomized multiplex CRISPRi-Seq approach for the identification of critical combinations of genes

Identifying virulence-critical genes from pathogens is often limited by functional redundancy. To rapidly interrogate the contributions of combinations of genes to a biological outcome, we have developed a multiplex, randomized CRISPR interference sequencing (MuRCiS) approach. At its center is a new...

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Bibliographic Details
Main Authors: Nicole A Ellis, Kevin S Myers, Jessica Tung, Anne Davidson Ward, Kathryn Johnston, Katherine E Bonnington, Timothy J Donohue, Matthias P Machner
Format: Article
Language:English
Published: eLife Sciences Publications Ltd 2023-12-01
Series:eLife
Subjects:
Legionella pneumophila
intracellular pathogen
translocated effectors
Online Access:https://elifesciences.org/articles/86903

Internet

https://elifesciences.org/articles/86903

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