In vitro EVALUATION OF HYDROALCOHOLIC EXTRACTS OF MYCELIUM, BASIDIOMATA AND SPENT SUBSTRATE OF Pleurotus ostreatus AGAINST Haemonchus contortus
Background. The edible mushroom Pleurotus ostreatus is a potential alternative for the control of gastrointestinal nematodes due to their nematoxins and secondary metabolites. Objective. To evaluate the in vitro anthelmintic activity of hydroalcoholic extracts of mycelium, basidiomata and spent subs...
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| Format: | Article |
| Language: | English |
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Universidad Autónoma de Yucatán
2021-04-01
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| Series: | Tropical and Subtropical Agroecosystems |
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| Online Access: | https://www.revista.ccba.uady.mx/ojs/index.php/TSA/article/view/3272 |
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| author | Luis Alfredo Valdez-Uriostegui Alma Delia Sánchez-García Alejandro Zamilpa Jose Ernesto Sánchez Roberto González-Garduño Pedro Mendoza-de-Gives Gloria Sarahi Castañeda-Ramirez Manases González-Cortázar Liliana Aguilar-Marcelino |
| author_facet | Luis Alfredo Valdez-Uriostegui Alma Delia Sánchez-García Alejandro Zamilpa Jose Ernesto Sánchez Roberto González-Garduño Pedro Mendoza-de-Gives Gloria Sarahi Castañeda-Ramirez Manases González-Cortázar Liliana Aguilar-Marcelino |
| author_sort | Luis Alfredo Valdez-Uriostegui |
| collection | DOAJ |
| description | Background. The edible mushroom Pleurotus ostreatus is a potential alternative for the control of gastrointestinal nematodes due to their nematoxins and secondary metabolites. Objective. To evaluate the in vitro anthelmintic activity of hydroalcoholic extracts of mycelium, basidiomata and spent substrate of the edible mushroom P. ostreatus against eggs and unsheathed larvae of Haemonchus contortus. Methodology. The mycelium of P. ostreatus was extracted with ethanol–water (70:30) and the basidiomata and the spent substrate with methanol–water (70:30) which were concentrated to obtain the mycelium (MHA), basidiomata (BHA) and spent mushroom substrate extract (SMSE) extracts. The eggs and larvae of H. contortus were exposed to different extracts in 96-well plates. The tests used were the egg-hatch and larval mortality tests. Three exposure times, 24, 48 and 72 h, were considered. The data were analyzed with a factorial design and the comparison of means by Duncan test. Results. The percentage anthelmintic effectiveness of the mycelium hydroalcoholic extract (MHA) of P. ostreatus against eggs and larvae of H. contortus were 93 and 96.8% at 12.8 and 240 mg/mL (respectively) after 72 h. Also, activity was observed for SMSE and BHA against eggs, with values of 87.2 and 100%, respectively, at 25.6 mg/mL after 72 h. However, SMSE showed no activity against H. contortus larvae, while the BHA extract showed less than 70% corresponding activity. Implications. This was an exploratory study of in vitro tests; the in vivo studies are still needed. Conclusion. The MHA extract of P. ostreatus showed anthelmintic activity against the eggs and larvae of H. contortus. |
| first_indexed | 2024-12-23T05:25:23Z |
| format | Article |
| id | doaj.art-f143705b5a9c477ea37d7844726a74d3 |
| institution | Directory Open Access Journal |
| issn | 1870-0462 |
| language | English |
| last_indexed | 2024-12-23T05:25:23Z |
| publishDate | 2021-04-01 |
| publisher | Universidad Autónoma de Yucatán |
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| series | Tropical and Subtropical Agroecosystems |
| spelling | doaj.art-f143705b5a9c477ea37d7844726a74d32022-12-21T17:58:37ZengUniversidad Autónoma de YucatánTropical and Subtropical Agroecosystems1870-04622021-04-0124210.56369/tsaes.32721110In vitro EVALUATION OF HYDROALCOHOLIC EXTRACTS OF MYCELIUM, BASIDIOMATA AND SPENT SUBSTRATE OF Pleurotus ostreatus AGAINST Haemonchus contortusLuis Alfredo Valdez-Uriostegui0Alma Delia Sánchez-García1Alejandro Zamilpa2Jose Ernesto Sánchez3Roberto González-Garduño4Pedro Mendoza-de-Gives5Gloria Sarahi Castañeda-Ramirez6Manases González-Cortázar7Liliana Aguilar-Marcelino8Area de Helmintología, Centro Nacional de Investigación Disciplinaria en Salud Animal e Inocuidad Parasitología Veterinaria, INIFAP, Carretera Federal Cuernavaca-Cuautla No. 8534, Col. Progreso, C.P. 62550, Jiutepec, Morelos, México.Universidad Politécnica del Estado de Morelos. Boulevard Cuauhnáhuac #566, Col. Lomas del Texcal, C.P. 62550. Jiutepec, Morelos. México.Centro de Investigaciones Biomédicas del Sur, Instituto Mexicano del Seguro Social, Argentina No. 1. Col. Centro, C.P. 62790, Xochitepec, Morelos, México.El Colegio de la Frontera Sur, Apdo. Postal 36, C.P. 30700, Tapachula, Chiapas, MéxicoUniversidad Autónoma Chapingo, Unidad Regional Universitaria Sursureste, km 7.5 Carretera Teapa-Vicente Guerrero, Teapa, Tabasco, MéxicoArea de Helmintología, Centro Nacional de Investigación Disciplinaria en Salud Animal e Inocuidad Parasitología Veterinaria, INIFAP, Carretera Federal Cuernavaca-Cuautla No. 8534, Col. Progreso, C.P. 62550, Jiutepec, Morelos, México.Area de Helmintología, Centro Nacional de Investigación Disciplinaria en Salud Animal e Inocuidad Parasitología Veterinaria, INIFAP, Carretera Federal Cuernavaca-Cuautla No. 8534, Col. Progreso, C.P. 62550, Jiutepec, Morelos, México.Centro de Investigaciones Biomédicas del Sur, Instituto Mexicano del Seguro Social, Argentina No. 1. Col. Centro, C.P. 62790, Xochitepec, Morelos, México.Dra. Liliana Aguilar Marcelino Researcher at the Department of Helminthology. CENID-ANIMAL SALUD Y SEGURIDAD, INIFAP. Federal Highway Cuernavaca-Cuautla No. 8534 Colony Progreso, Jiutepec, Morelos, Mexico. C.P. 62550 Telephones: + 52-777-319-28-60, ext. Office: 121 and Laboratory Ext.: 136. IP: 01 800 088 2222 (0155) 55 387 18 700 Ext. Office: 80419 http://ampave.org/index.php/consejo-directiveBackground. The edible mushroom Pleurotus ostreatus is a potential alternative for the control of gastrointestinal nematodes due to their nematoxins and secondary metabolites. Objective. To evaluate the in vitro anthelmintic activity of hydroalcoholic extracts of mycelium, basidiomata and spent substrate of the edible mushroom P. ostreatus against eggs and unsheathed larvae of Haemonchus contortus. Methodology. The mycelium of P. ostreatus was extracted with ethanol–water (70:30) and the basidiomata and the spent substrate with methanol–water (70:30) which were concentrated to obtain the mycelium (MHA), basidiomata (BHA) and spent mushroom substrate extract (SMSE) extracts. The eggs and larvae of H. contortus were exposed to different extracts in 96-well plates. The tests used were the egg-hatch and larval mortality tests. Three exposure times, 24, 48 and 72 h, were considered. The data were analyzed with a factorial design and the comparison of means by Duncan test. Results. The percentage anthelmintic effectiveness of the mycelium hydroalcoholic extract (MHA) of P. ostreatus against eggs and larvae of H. contortus were 93 and 96.8% at 12.8 and 240 mg/mL (respectively) after 72 h. Also, activity was observed for SMSE and BHA against eggs, with values of 87.2 and 100%, respectively, at 25.6 mg/mL after 72 h. However, SMSE showed no activity against H. contortus larvae, while the BHA extract showed less than 70% corresponding activity. Implications. This was an exploratory study of in vitro tests; the in vivo studies are still needed. Conclusion. The MHA extract of P. ostreatus showed anthelmintic activity against the eggs and larvae of H. contortus.https://www.revista.ccba.uady.mx/ojs/index.php/TSA/article/view/3272anthelminticextractshaemonchus contortuspleurotus ostreatus. |
| spellingShingle | Luis Alfredo Valdez-Uriostegui Alma Delia Sánchez-García Alejandro Zamilpa Jose Ernesto Sánchez Roberto González-Garduño Pedro Mendoza-de-Gives Gloria Sarahi Castañeda-Ramirez Manases González-Cortázar Liliana Aguilar-Marcelino In vitro EVALUATION OF HYDROALCOHOLIC EXTRACTS OF MYCELIUM, BASIDIOMATA AND SPENT SUBSTRATE OF Pleurotus ostreatus AGAINST Haemonchus contortus Tropical and Subtropical Agroecosystems anthelmintic extracts haemonchus contortus pleurotus ostreatus. |
| title | In vitro EVALUATION OF HYDROALCOHOLIC EXTRACTS OF MYCELIUM, BASIDIOMATA AND SPENT SUBSTRATE OF Pleurotus ostreatus AGAINST Haemonchus contortus |
| title_full | In vitro EVALUATION OF HYDROALCOHOLIC EXTRACTS OF MYCELIUM, BASIDIOMATA AND SPENT SUBSTRATE OF Pleurotus ostreatus AGAINST Haemonchus contortus |
| title_fullStr | In vitro EVALUATION OF HYDROALCOHOLIC EXTRACTS OF MYCELIUM, BASIDIOMATA AND SPENT SUBSTRATE OF Pleurotus ostreatus AGAINST Haemonchus contortus |
| title_full_unstemmed | In vitro EVALUATION OF HYDROALCOHOLIC EXTRACTS OF MYCELIUM, BASIDIOMATA AND SPENT SUBSTRATE OF Pleurotus ostreatus AGAINST Haemonchus contortus |
| title_short | In vitro EVALUATION OF HYDROALCOHOLIC EXTRACTS OF MYCELIUM, BASIDIOMATA AND SPENT SUBSTRATE OF Pleurotus ostreatus AGAINST Haemonchus contortus |
| title_sort | in vitro evaluation of hydroalcoholic extracts of mycelium basidiomata and spent substrate of pleurotus ostreatus against haemonchus contortus |
| topic | anthelmintic extracts haemonchus contortus pleurotus ostreatus. |
| url | https://www.revista.ccba.uady.mx/ojs/index.php/TSA/article/view/3272 |
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