Reduced polyfunctional T cells and increased cellular activation markers in adult allergy patients reporting adverse reactions to food
Abstract Background The underlying cellular mechanisms causing adverse reactions to food are complex and still not fully understood. Therefore, in this study we aimed to identify functional and/or phenotypical immune cell signatures characteristic for adult patients reporting adverse reactions to fo...
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| Format: | Article |
| Language: | English |
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BMC
2020-07-01
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| Series: | BMC Immunology |
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| Online Access: | http://link.springer.com/article/10.1186/s12865-020-00373-w |
| _version_ | 1828751996873605120 |
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| author | Friederike Sonnet Ellen Namork Eva Stylianou Ingvild Gaare-Olstad Kanutte Huse Sandra Andorf Siri Mjaaland Hubert Dirven Unni Nygaard |
| author_facet | Friederike Sonnet Ellen Namork Eva Stylianou Ingvild Gaare-Olstad Kanutte Huse Sandra Andorf Siri Mjaaland Hubert Dirven Unni Nygaard |
| author_sort | Friederike Sonnet |
| collection | DOAJ |
| description | Abstract Background The underlying cellular mechanisms causing adverse reactions to food are complex and still not fully understood. Therefore, in this study we aimed to identify functional and/or phenotypical immune cell signatures characteristic for adult patients reporting adverse reactions to food. By mass cytometry, we performed high-dimensional profiling of peripheral blood mononuclear cells (PBMC) from adult patients reporting adverse reactions to food and healthy controls. The patients were grouped according to sIgE-positive or sIgE-negative serology to common food and inhalant allergens. Two broad antibody panels were used, allowing determination of major immune cell populations in PBMC, as well as activation status, proliferation status, and cytokine expression patterns after PMA/ionomycin-stimulation on a single cell level. Results By use of data-driven algorithms, several cell populations were identified showing significantly different marker expression between the groups. Most striking was an impaired frequency and function of polyfunctional CD4+ and CD8+ T cells in patients reporting adverse reactions to food compared to the controls. Further, subpopulations of monocytes, T cells, and B cells had increased expression of functional markers such as CD371, CD69, CD25, CD28, and/or HLA-DR as well as decreased expression of CD23 in the patients. Most of the differing cell subpopulations were similarly altered in the two subgroups of patients. Conclusion Our results suggest common immune cell features for both patient subgroups reporting adverse reactions to food, and provide a basis for further studies on mechanistic and diagnostic biomarker studies in food allergy. |
| first_indexed | 2024-12-10T21:02:46Z |
| format | Article |
| id | doaj.art-f170826615c34af19060c968ea4fb44a |
| institution | Directory Open Access Journal |
| issn | 1471-2172 |
| language | English |
| last_indexed | 2024-12-10T21:02:46Z |
| publishDate | 2020-07-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Immunology |
| spelling | doaj.art-f170826615c34af19060c968ea4fb44a2022-12-22T01:33:45ZengBMCBMC Immunology1471-21722020-07-0121111410.1186/s12865-020-00373-wReduced polyfunctional T cells and increased cellular activation markers in adult allergy patients reporting adverse reactions to foodFriederike Sonnet0Ellen Namork1Eva Stylianou2Ingvild Gaare-Olstad3Kanutte Huse4Sandra Andorf5Siri Mjaaland6Hubert Dirven7Unni Nygaard8Department of Toxicology and Risk Assessment, Norwegian Institute of Public HealthDepartment of Toxicology and Risk Assessment, Norwegian Institute of Public HealthRegional Unit for Asthma, Allergy and Hypersensitivity, Department of Pulmonary Diseases, Oslo University HospitalRegional Unit for Asthma, Allergy and Hypersensitivity, Department of Pulmonary Diseases, Oslo University HospitalDepartment of Cancer Immunology, Oslo University HospitalSean N. Parker Center for Allergy and Asthma Research, Stanford University School of MedicineDepartment of Infectious Diseases Epidemiology and Modelling, Norwegian Institute of Public HealthDepartment of Toxicology and Risk Assessment, Norwegian Institute of Public HealthDepartment of Toxicology and Risk Assessment, Norwegian Institute of Public HealthAbstract Background The underlying cellular mechanisms causing adverse reactions to food are complex and still not fully understood. Therefore, in this study we aimed to identify functional and/or phenotypical immune cell signatures characteristic for adult patients reporting adverse reactions to food. By mass cytometry, we performed high-dimensional profiling of peripheral blood mononuclear cells (PBMC) from adult patients reporting adverse reactions to food and healthy controls. The patients were grouped according to sIgE-positive or sIgE-negative serology to common food and inhalant allergens. Two broad antibody panels were used, allowing determination of major immune cell populations in PBMC, as well as activation status, proliferation status, and cytokine expression patterns after PMA/ionomycin-stimulation on a single cell level. Results By use of data-driven algorithms, several cell populations were identified showing significantly different marker expression between the groups. Most striking was an impaired frequency and function of polyfunctional CD4+ and CD8+ T cells in patients reporting adverse reactions to food compared to the controls. Further, subpopulations of monocytes, T cells, and B cells had increased expression of functional markers such as CD371, CD69, CD25, CD28, and/or HLA-DR as well as decreased expression of CD23 in the patients. Most of the differing cell subpopulations were similarly altered in the two subgroups of patients. Conclusion Our results suggest common immune cell features for both patient subgroups reporting adverse reactions to food, and provide a basis for further studies on mechanistic and diagnostic biomarker studies in food allergy.http://link.springer.com/article/10.1186/s12865-020-00373-wFood allergyAdverse reactions to foodSpecific IgEPolyfunctional cellsCyTOF/mass cytometryCITRUS algorithm |
| spellingShingle | Friederike Sonnet Ellen Namork Eva Stylianou Ingvild Gaare-Olstad Kanutte Huse Sandra Andorf Siri Mjaaland Hubert Dirven Unni Nygaard Reduced polyfunctional T cells and increased cellular activation markers in adult allergy patients reporting adverse reactions to food BMC Immunology Food allergy Adverse reactions to food Specific IgE Polyfunctional cells CyTOF/mass cytometry CITRUS algorithm |
| title | Reduced polyfunctional T cells and increased cellular activation markers in adult allergy patients reporting adverse reactions to food |
| title_full | Reduced polyfunctional T cells and increased cellular activation markers in adult allergy patients reporting adverse reactions to food |
| title_fullStr | Reduced polyfunctional T cells and increased cellular activation markers in adult allergy patients reporting adverse reactions to food |
| title_full_unstemmed | Reduced polyfunctional T cells and increased cellular activation markers in adult allergy patients reporting adverse reactions to food |
| title_short | Reduced polyfunctional T cells and increased cellular activation markers in adult allergy patients reporting adverse reactions to food |
| title_sort | reduced polyfunctional t cells and increased cellular activation markers in adult allergy patients reporting adverse reactions to food |
| topic | Food allergy Adverse reactions to food Specific IgE Polyfunctional cells CyTOF/mass cytometry CITRUS algorithm |
| url | http://link.springer.com/article/10.1186/s12865-020-00373-w |
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