基于酶反应动力学理论优化脂肪酶活力测定体系Optimization of lipase activity assay system based on enzyme reaction kinetics theory
为了提高棕榈酸对硝基苯酯(p-NPP)法测定脂肪酶活力的灵敏度和准确度,以来源于Rhizopus oryzae的脂肪酶Lipase F-AP 15为研究对象,首先对其最适反应pH和反应温度进行优化,然后将表面活性剂作为激活因子引入酶活力测定体系,探究影响机制,并依据酶反应动力学理论优化测定条件(底物浓度、酶质量浓度、反应时间),对优化的p-NPP法的重复性和准确性进行了检验,最后以另一个来源的脂肪酶Lipase-PPL为模型,以同样思路与方法改进与优化其酶活力测定体系,以考察优化方法和思路的适用性。结果表明:表面活性剂PEG 8000的引入使得覆盖在脂肪酶活性位点的α-螺旋结构被重排而打开,脂...
Main Author: | 张帆1,2,程路峰1,曹红2,何德飞3,郑兰兰2,李春4 ZHANG Fan1,2, CHENG Lufeng1, CAO Hong2, HE Defei3, ZHENG Lanlan2, LI Chun4 |
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Format: | Article |
Language: | English |
Published: |
中粮工科(西安)国际工程有限公司
2023-05-01
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Series: | Zhongguo youzhi |
Subjects: | |
Online Access: | http://tg.chinaoils.cn/ch/reader/create_pdf.aspx?file_no=20230528&year_id=2023&quarter_id=5&falg=1 |
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