In vitro differentiation of Trypanosoma cruzi epimastigotes into metacyclic trypomastigotes using a biphasic medium

In vitro differentiation of Trypanosoma cruzi epimastigotes into metacyclic trypomastigotes using a biphasic medium

Summary: The pathogen Trypanosoma cruzi differentiates from epimastigotes (E) into infective metacyclic trypomastigotes (MTs) to invade the mammalian cell. This process, called metacyclogenesis, is mimicked in vitro by nutrient starvation or incubation with minimal media. Here, we describe an altern...

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Bibliographic Details
Main Authors: Jessica Rodríguez Durán, Arturo Muñoz-Calderón, Karina Andrea Gómez, Mariana Potenza
Format: Article
Language:English
Published: Elsevier 2021-09-01
Series:STAR Protocols
Subjects:
Cell culture
Cell separation/fractionation
Microbiology
Model Organisms
Cell Differentiation
Online Access:http://www.sciencedirect.com/science/article/pii/S266616672100410X

Internet

http://www.sciencedirect.com/science/article/pii/S266616672100410X

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