CTLA-4 expressed by human dendritic cells modulates thei cytokine secretion and induction of T cell proliferation
<p>CTLA-4 is the major nefative regulator of T cell response. We have analyzed the expression of CTLA-4 in human monocytes and monocyte-derived DCs and the effects of its engagement on cytokine production and T cell stimulatory activity by mature DCs (mDCs). We found the CTLA-4 was highly expr...
| Main Authors: | , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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PAGEPress Publications
2011-01-01
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| Series: | Journal of Biological Research |
| Subjects: | |
| Online Access: | http://www.pagepressjournals.org/index.php/jbr/article/view/4651 |
| _version_ | 1811322259680264192 |
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| author | S. Laurent P. Carrega D. Saverino P. Piccioli M. Camoriamo A. Morabito B. Dozin V. Fontana R. Simone L. Mortara M.C. Mingari G. Ferlazzo M.P. Pistillo |
| author_facet | S. Laurent P. Carrega D. Saverino P. Piccioli M. Camoriamo A. Morabito B. Dozin V. Fontana R. Simone L. Mortara M.C. Mingari G. Ferlazzo M.P. Pistillo |
| author_sort | S. Laurent |
| collection | DOAJ |
| description | <p>CTLA-4 is the major nefative regulator of T cell response. We have analyzed the expression of CTLA-4 in human monocytes and monocyte-derived DCs and the effects of its engagement on cytokine production and T cell stimulatory activity by mature DCs (mDCs). We found the CTLA-4 was highly expressed on freshly isolated monocytes, then down-modulated on the immature DCs (iDCs) and upregulated on mDCs. Treatment of mDCS with an agonistic anti-CTLA-4 m Ab enhanced secretion of IL-10 but reduced secretion of IL-8 and IL-12, as well as autologous CD4* T-cell proliferation in response to simulation with PPD recall antigenloaded-DCs. Neutralization of IL-10 with an anti-IL-10 antibody partially restored the ability of anti-CTLA-4-treated mDCs to stimulate T cell proliferation in response to PPD. Our data provide the first evidence that CTLA-4 receptor is expressed by human mDCs and exerts immune modulatory effects in these cells.</p> |
| first_indexed | 2024-04-13T13:32:04Z |
| format | Article |
| id | doaj.art-f1a070d8c9ba4f86b9adff424cebd24a |
| institution | Directory Open Access Journal |
| issn | 1826-8838 2284-0230 |
| language | English |
| last_indexed | 2024-04-13T13:32:04Z |
| publishDate | 2011-01-01 |
| publisher | PAGEPress Publications |
| record_format | Article |
| series | Journal of Biological Research |
| spelling | doaj.art-f1a070d8c9ba4f86b9adff424cebd24a2022-12-22T02:44:55ZengPAGEPress PublicationsJournal of Biological Research1826-88382284-02302011-01-0184110.4081/jbr.2011.46513800CTLA-4 expressed by human dendritic cells modulates thei cytokine secretion and induction of T cell proliferationS. LaurentP. CarregaD. SaverinoP. PiccioliM. CamoriamoA. MorabitoB. DozinV. FontanaR. SimoneL. MortaraM.C. MingariG. FerlazzoM.P. Pistillo<p>CTLA-4 is the major nefative regulator of T cell response. We have analyzed the expression of CTLA-4 in human monocytes and monocyte-derived DCs and the effects of its engagement on cytokine production and T cell stimulatory activity by mature DCs (mDCs). We found the CTLA-4 was highly expressed on freshly isolated monocytes, then down-modulated on the immature DCs (iDCs) and upregulated on mDCs. Treatment of mDCS with an agonistic anti-CTLA-4 m Ab enhanced secretion of IL-10 but reduced secretion of IL-8 and IL-12, as well as autologous CD4* T-cell proliferation in response to simulation with PPD recall antigenloaded-DCs. Neutralization of IL-10 with an anti-IL-10 antibody partially restored the ability of anti-CTLA-4-treated mDCs to stimulate T cell proliferation in response to PPD. Our data provide the first evidence that CTLA-4 receptor is expressed by human mDCs and exerts immune modulatory effects in these cells.</p>http://www.pagepressjournals.org/index.php/jbr/article/view/4651CTLA-4, DC, cytokine secretion, T cell proliferation |
| spellingShingle | S. Laurent P. Carrega D. Saverino P. Piccioli M. Camoriamo A. Morabito B. Dozin V. Fontana R. Simone L. Mortara M.C. Mingari G. Ferlazzo M.P. Pistillo CTLA-4 expressed by human dendritic cells modulates thei cytokine secretion and induction of T cell proliferation Journal of Biological Research CTLA-4, DC, cytokine secretion, T cell proliferation |
| title | CTLA-4 expressed by human dendritic cells modulates thei cytokine secretion and induction of T cell proliferation |
| title_full | CTLA-4 expressed by human dendritic cells modulates thei cytokine secretion and induction of T cell proliferation |
| title_fullStr | CTLA-4 expressed by human dendritic cells modulates thei cytokine secretion and induction of T cell proliferation |
| title_full_unstemmed | CTLA-4 expressed by human dendritic cells modulates thei cytokine secretion and induction of T cell proliferation |
| title_short | CTLA-4 expressed by human dendritic cells modulates thei cytokine secretion and induction of T cell proliferation |
| title_sort | ctla 4 expressed by human dendritic cells modulates thei cytokine secretion and induction of t cell proliferation |
| topic | CTLA-4, DC, cytokine secretion, T cell proliferation |
| url | http://www.pagepressjournals.org/index.php/jbr/article/view/4651 |
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