High-content analysis of neuronal morphology in human iPSC-derived neurons

High-content analysis of neuronal morphology in human iPSC-derived neurons

Summary: We present a high-content analysis (HCA) protocol for monitoring the outgrowth capacity of human neurons derived from induced pluripotent stem cells (iPSCs). We describe steps to perform HCA imaging, followed by quantifying the morphology of dendrites and axons within a high-throughput syst...

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Bibliographic Details
Main Authors: Selene Lickfett, Carmen Menacho, Annika Zink, Narasimha Swamy Telugu, Mathias Beller, Sebastian Diecke, Sidney Cambridge, Alessandro Prigione
Format: Article
Language:English
Published: Elsevier 2022-09-01
Series:STAR Protocols
Subjects:
High Throughput Screening
Microscopy
Neuroscience
Stem Cells
Cell Differentiation
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166722004476

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http://www.sciencedirect.com/science/article/pii/S2666166722004476

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