Enhanced BRET technology for the monitoring of agonist-induced and agonist-independent interactions between GPCRs and β-arrestins

Enhanced BRET technology for the monitoring of agonist-induced and agonist-independent interactions between GPCRs and β-arrestins

The bioluminescence resonance energy transfer (BRET) technique has become extremely valuable for the real-time monitoring of protein-protein interactions in live cells. This method is highly amenable to the detection of G protein-coupled receptor (GPCR) interactions with proteins critical for regula...

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Bibliographic Details
Main Authors: Martina eKocan, Matthew eDalrymple, Ruth eSeeber, Brian eFeldman, Kevin ePfleger
Format: Article
Language:English
Published: Frontiers Media S.A. 2011-01-01
Series:Frontiers in Endocrinology
Subjects:
Arrestin
vasopressin
BRET
bioluminescence resonance energy transfer
GPCR
orexin
Online Access:http://journal.frontiersin.org/Journal/10.3389/fendo.2010.00012/full

Internet

http://journal.frontiersin.org/Journal/10.3389/fendo.2010.00012/full

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