From Enzyme to Preparative Cascade Reactions with Immobilized Enzymes: Tuning Fe(II)/α-Ketoglutarate-Dependent Lysine Hydroxylases for Application in Biotransformations

From Enzyme to Preparative Cascade Reactions with Immobilized Enzymes: Tuning Fe(II)/α-Ketoglutarate-Dependent Lysine Hydroxylases for Application in Biotransformations

Fe(II)/α-ketoglutarate-dependent dioxygenases (KDOs) catalyze a broad range of selective C–H oxidation reactions. However, the difficult production of KDOs in recombinant <i>E. coli</i> strains and their instability in purified form have so far limited their application in preparative bi...

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Bibliographic Details
Main Authors: Selina Seide, Lilia Arnold, Solange Wetzels, Mariela Bregu, Jochem Gätgens, Martina Pohl
Format: Article
Language:English
Published: MDPI AG 2022-03-01
Series:Catalysts
Subjects:
2-oxoglutarate-dependent oxygenases
hydroxylation
amino acid modification
1,5-diamino pentane
1,5-diaminopentane-(2<i>S</i>)-ol
L-ornithine
Online Access:https://www.mdpi.com/2073-4344/12/4/354

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https://www.mdpi.com/2073-4344/12/4/354

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