Enumeration of Mycobacterium leprae using real-time PCR.

Enumeration of Mycobacterium leprae using real-time PCR.

Mycobacterium leprae is not cultivable in axenic media, and direct microscopic enumeration of the bacilli is complex, labor intensive, and suffers from limited sensitivity and specificity. We have developed a real-time PCR assay for quantifying M. leprae DNA in biological samples. Primers were ident...

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Bibliographic Details
Main Authors: Richard W Truman, P Kyle Andrews, Naoko Y Robbins, Linda B Adams, James L Krahenbuhl, Thomas P Gillis
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2008-01-01
Series:PLoS Neglected Tropical Diseases
Online Access:http://europepmc.org/articles/PMC2570796?pdf=render

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http://europepmc.org/articles/PMC2570796?pdf=render

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