Epitranscriptomic mapping of RNA modifications at single-nucleotide resolution using rhodamine sequencing (Rho-seq)

Epitranscriptomic mapping of RNA modifications at single-nucleotide resolution using rhodamine sequencing (Rho-seq)

Summary: The recent development of epitranscriptomics revealed a new fundamental layer of gene expression, but the mapping of most RNA modifications remains technically challenging. Here, we describe our protocol for Rho-Seq, which enables the mapping of dihydrouridine RNA modification at single-nuc...

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Bibliographic Details
Main Authors: Olivier Finet, Carlo Yague-Sanz, Damien Hermand
Format: Article
Language:English
Published: Elsevier 2022-06-01
Series:STAR Protocols
Subjects:
Sequence analysis
RNAseq
Molecular Biology
Gene Expression
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166722002490

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http://www.sciencedirect.com/science/article/pii/S2666166722002490

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