Pf Filamentous Phage Requires UvrD for Replication in <named-content content-type="genus-species">Pseudomonas aeruginosa</named-content>
ABSTRACT Pf is a lysogenic filamentous phage that promotes biofilm development in Pseudomonas aeruginosa. Pf replicates by a rolling circle replication system which depends on a phage-encoded initiator protein and host factors usually involved in chromosome replication. Rep, an accessory replicative...
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Format: | Article |
Language: | English |
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American Society for Microbiology
2016-02-01
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Series: | mSphere |
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Online Access: | https://journals.asm.org/doi/10.1128/mSphere.00104-15 |
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author | Eriel Martínez Javier Campos-Gómez |
author_facet | Eriel Martínez Javier Campos-Gómez |
author_sort | Eriel Martínez |
collection | DOAJ |
description | ABSTRACT Pf is a lysogenic filamentous phage that promotes biofilm development in Pseudomonas aeruginosa. Pf replicates by a rolling circle replication system which depends on a phage-encoded initiator protein and host factors usually involved in chromosome replication. Rep, an accessory replicative DNA helicase, is crucial for replication of filamentous phages in Escherichia coli. In contrast, here we show that, instead of depending on Rep, Pf replication depends on UvrD, an accessory helicase implicated in DNA repair. In this study, we also identified the initiator protein of Pf and found that it shares similarities with that of Vibrio phages CTXφ and VGJφ, which also depend on UvrD for replication. A structural comparative analysis of the initiator proteins of most known filamentous phages described thus far suggested that UvrD, known as a nonreplicative helicase, is involved in rolling circle replication of filamentous phages in diverse bacteria genera. This report consolidates knowledge on the new role of UvrD in filamentous phage replication, a function previously thought to be exclusive of Rep helicase. IMPORTANCE Biofilm development is a key component of the ability of Pseudomonas aeruginosa to evade host immune defenses and resist multiple drugs. Induction of the filamentous phage Pf, which usually is lysogenized in clinical and environmental isolates of P. aeruginosa, plays an important role in biofilm assembly, maturation, and dispersal. Despite the clinical relevance of Pf, the molecular biology of this phage is largely unknown. In this study, we found that rolling circle replication of Pf depends on UvrD, a DNA helicase normally involved in DNA repair. We also identified the initiator protein of Pf and found that it shares structural similarity with that of Vibrio cholerae phages CTXφ and VGJφ, which also use UvrD for replication. Our results reveal that, in addition to DNA repair, UvrD plays an essential role in rolling circle replication of filamentous phages among diverse bacteria genera, adding a new, previously unrecognized function of this accessory helicase. |
first_indexed | 2024-12-19T02:40:50Z |
format | Article |
id | doaj.art-f1ded1e8bf264090a51912a9e970defa |
institution | Directory Open Access Journal |
issn | 2379-5042 |
language | English |
last_indexed | 2024-12-19T02:40:50Z |
publishDate | 2016-02-01 |
publisher | American Society for Microbiology |
record_format | Article |
series | mSphere |
spelling | doaj.art-f1ded1e8bf264090a51912a9e970defa2022-12-21T20:39:08ZengAmerican Society for MicrobiologymSphere2379-50422016-02-011110.1128/mSphere.00104-15Pf Filamentous Phage Requires UvrD for Replication in <named-content content-type="genus-species">Pseudomonas aeruginosa</named-content>Eriel Martínez0Javier Campos-Gómez1Department of Infectious Diseases, Drug Discovery Division, Southern Research, Birmingham, Alabama, USADepartment of Infectious Diseases, Drug Discovery Division, Southern Research, Birmingham, Alabama, USAABSTRACT Pf is a lysogenic filamentous phage that promotes biofilm development in Pseudomonas aeruginosa. Pf replicates by a rolling circle replication system which depends on a phage-encoded initiator protein and host factors usually involved in chromosome replication. Rep, an accessory replicative DNA helicase, is crucial for replication of filamentous phages in Escherichia coli. In contrast, here we show that, instead of depending on Rep, Pf replication depends on UvrD, an accessory helicase implicated in DNA repair. In this study, we also identified the initiator protein of Pf and found that it shares similarities with that of Vibrio phages CTXφ and VGJφ, which also depend on UvrD for replication. A structural comparative analysis of the initiator proteins of most known filamentous phages described thus far suggested that UvrD, known as a nonreplicative helicase, is involved in rolling circle replication of filamentous phages in diverse bacteria genera. This report consolidates knowledge on the new role of UvrD in filamentous phage replication, a function previously thought to be exclusive of Rep helicase. IMPORTANCE Biofilm development is a key component of the ability of Pseudomonas aeruginosa to evade host immune defenses and resist multiple drugs. Induction of the filamentous phage Pf, which usually is lysogenized in clinical and environmental isolates of P. aeruginosa, plays an important role in biofilm assembly, maturation, and dispersal. Despite the clinical relevance of Pf, the molecular biology of this phage is largely unknown. In this study, we found that rolling circle replication of Pf depends on UvrD, a DNA helicase normally involved in DNA repair. We also identified the initiator protein of Pf and found that it shares structural similarity with that of Vibrio cholerae phages CTXφ and VGJφ, which also use UvrD for replication. Our results reveal that, in addition to DNA repair, UvrD plays an essential role in rolling circle replication of filamentous phages among diverse bacteria genera, adding a new, previously unrecognized function of this accessory helicase.https://journals.asm.org/doi/10.1128/mSphere.00104-15Pseudomonas aeruginosaPf filamentous phageUvrD helicaseRep helicasehistone-like HUrolling circle replication |
spellingShingle | Eriel Martínez Javier Campos-Gómez Pf Filamentous Phage Requires UvrD for Replication in <named-content content-type="genus-species">Pseudomonas aeruginosa</named-content> mSphere Pseudomonas aeruginosa Pf filamentous phage UvrD helicase Rep helicase histone-like HU rolling circle replication |
title | Pf Filamentous Phage Requires UvrD for Replication in <named-content content-type="genus-species">Pseudomonas aeruginosa</named-content> |
title_full | Pf Filamentous Phage Requires UvrD for Replication in <named-content content-type="genus-species">Pseudomonas aeruginosa</named-content> |
title_fullStr | Pf Filamentous Phage Requires UvrD for Replication in <named-content content-type="genus-species">Pseudomonas aeruginosa</named-content> |
title_full_unstemmed | Pf Filamentous Phage Requires UvrD for Replication in <named-content content-type="genus-species">Pseudomonas aeruginosa</named-content> |
title_short | Pf Filamentous Phage Requires UvrD for Replication in <named-content content-type="genus-species">Pseudomonas aeruginosa</named-content> |
title_sort | pf filamentous phage requires uvrd for replication in named content content type genus species pseudomonas aeruginosa named content |
topic | Pseudomonas aeruginosa Pf filamentous phage UvrD helicase Rep helicase histone-like HU rolling circle replication |
url | https://journals.asm.org/doi/10.1128/mSphere.00104-15 |
work_keys_str_mv | AT erielmartinez pffilamentousphagerequiresuvrdforreplicationinnamedcontentcontenttypegenusspeciespseudomonasaeruginosanamedcontent AT javiercamposgomez pffilamentousphagerequiresuvrdforreplicationinnamedcontentcontenttypegenusspeciespseudomonasaeruginosanamedcontent |