Maternal Folic Acid Intake and Methylation Status of Genes Associated with Ventricular Septal Defects in Children: Case–Control Study
Background: DNA methylation is the best epigenetic mechanism for explaining the interactions between nutrients and genes involved in intrauterine growth and development programming. A possible contributor of methylation abnormalities to congenital heart disease is the folate methylation regulatory p...
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MDPI AG
2021-06-01
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| Series: | Nutrients |
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| Online Access: | https://www.mdpi.com/2072-6643/13/6/2071 |
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| author | Sandra M. González-Peña Geovana Calvo-Anguiano Laura E. Martínez-de-Villarreal Patricia R. Ancer-Rodríguez José J. Lugo-Trampe Donato Saldivar-Rodríguez María D. Hernández-Almaguer Melissa Calzada-Dávila Ligia S. Guerrero-Orjuela Luis D. Campos-Acevedo |
| author_facet | Sandra M. González-Peña Geovana Calvo-Anguiano Laura E. Martínez-de-Villarreal Patricia R. Ancer-Rodríguez José J. Lugo-Trampe Donato Saldivar-Rodríguez María D. Hernández-Almaguer Melissa Calzada-Dávila Ligia S. Guerrero-Orjuela Luis D. Campos-Acevedo |
| author_sort | Sandra M. González-Peña |
| collection | DOAJ |
| description | Background: DNA methylation is the best epigenetic mechanism for explaining the interactions between nutrients and genes involved in intrauterine growth and development programming. A possible contributor of methylation abnormalities to congenital heart disease is the folate methylation regulatory pathway; however, the mechanisms and methylation patterns of VSD-associated genes are not fully understood. Objective: To determine if maternal dietary intake of folic acid (FA) is related to the methylation status (MS) of VSD-associated genes (AXIN1, MTHFR, TBX1, and TBX20). Methods: Prospective case–control study; 48 mothers and their children were evaluated. The mothers’ dietary variables were collected through a food frequency questionnaire focusing on FA and the consumption of supplements with FA. The MS of promoters of genes was determined in the children. Results: The intake of FA supplements was significantly higher in the control mothers. In terms of maternal folic acid consumption, significant differences were found in the first trimester of pregnancy. Significant differences were observed in the MS of MTHFR and AXIN1 genes in VSD and control children. A correlation between maternal FA supplementation and MS of AXIN1 and TBX20 genes was found in control and VSD children, respectively. Conclusions: A lower MS of AXIN1 genes and a higher MS of TBX20 genes is associated with FA maternal supplementation. |
| first_indexed | 2024-03-10T10:21:00Z |
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| issn | 2072-6643 |
| language | English |
| last_indexed | 2024-03-10T10:21:00Z |
| publishDate | 2021-06-01 |
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| record_format | Article |
| series | Nutrients |
| spelling | doaj.art-f1f1ecb40db74669aec9fd799260da8e2023-11-22T00:30:16ZengMDPI AGNutrients2072-66432021-06-01136207110.3390/nu13062071Maternal Folic Acid Intake and Methylation Status of Genes Associated with Ventricular Septal Defects in Children: Case–Control StudySandra M. González-Peña0Geovana Calvo-Anguiano1Laura E. Martínez-de-Villarreal2Patricia R. Ancer-Rodríguez3José J. Lugo-Trampe4Donato Saldivar-Rodríguez5María D. Hernández-Almaguer6Melissa Calzada-Dávila7Ligia S. Guerrero-Orjuela8Luis D. Campos-Acevedo9Clinical Nutrition, Hospital Universitario “Dr. José Eleuterio González” and Medicine Faculty, Autonomous University of Nuevo León, Monterrey 64460, MexicoGenetics Department, Hospital Universitario “Dr. José Eleuterio González” and Medicine Faculty, Autonomous University of Nuevo León, Monterrey 64460, MexicoGenetics Department, Hospital Universitario “Dr. José Eleuterio González” and Medicine Faculty, Autonomous University of Nuevo León, Monterrey 64460, MexicoClinical Nutrition, Hospital Universitario “Dr. José Eleuterio González” and Medicine Faculty, Autonomous University of Nuevo León, Monterrey 64460, MexicoGenetics Department, Hospital Universitario “Dr. José Eleuterio González” and Medicine Faculty, Autonomous University of Nuevo León, Monterrey 64460, MexicoGynecology and Obstetrics Department, Hospital Universitario “Dr. José Eleuterio González” and Medicine Faculty, Autonomous University of Nuevo León, Monterrey 64460, MexicoGenetics Department, Hospital Universitario “Dr. José Eleuterio González” and Medicine Faculty, Autonomous University of Nuevo León, Monterrey 64460, MexicoGenetics Department, Hospital Universitario “Dr. José Eleuterio González” and Medicine Faculty, Autonomous University of Nuevo León, Monterrey 64460, MexicoMedicine Faculty, National University of Colombia, Bogotá 111321, ColombiaGenetics Department, Hospital Universitario “Dr. José Eleuterio González” and Medicine Faculty, Autonomous University of Nuevo León, Monterrey 64460, MexicoBackground: DNA methylation is the best epigenetic mechanism for explaining the interactions between nutrients and genes involved in intrauterine growth and development programming. A possible contributor of methylation abnormalities to congenital heart disease is the folate methylation regulatory pathway; however, the mechanisms and methylation patterns of VSD-associated genes are not fully understood. Objective: To determine if maternal dietary intake of folic acid (FA) is related to the methylation status (MS) of VSD-associated genes (AXIN1, MTHFR, TBX1, and TBX20). Methods: Prospective case–control study; 48 mothers and their children were evaluated. The mothers’ dietary variables were collected through a food frequency questionnaire focusing on FA and the consumption of supplements with FA. The MS of promoters of genes was determined in the children. Results: The intake of FA supplements was significantly higher in the control mothers. In terms of maternal folic acid consumption, significant differences were found in the first trimester of pregnancy. Significant differences were observed in the MS of MTHFR and AXIN1 genes in VSD and control children. A correlation between maternal FA supplementation and MS of AXIN1 and TBX20 genes was found in control and VSD children, respectively. Conclusions: A lower MS of AXIN1 genes and a higher MS of TBX20 genes is associated with FA maternal supplementation.https://www.mdpi.com/2072-6643/13/6/2071folic acid intakeventricular septal defectscongenital heart diseasemethylation status<i>AXIN1</i><i>MTHFR</i> |
| spellingShingle | Sandra M. González-Peña Geovana Calvo-Anguiano Laura E. Martínez-de-Villarreal Patricia R. Ancer-Rodríguez José J. Lugo-Trampe Donato Saldivar-Rodríguez María D. Hernández-Almaguer Melissa Calzada-Dávila Ligia S. Guerrero-Orjuela Luis D. Campos-Acevedo Maternal Folic Acid Intake and Methylation Status of Genes Associated with Ventricular Septal Defects in Children: Case–Control Study Nutrients folic acid intake ventricular septal defects congenital heart disease methylation status <i>AXIN1</i> <i>MTHFR</i> |
| title | Maternal Folic Acid Intake and Methylation Status of Genes Associated with Ventricular Septal Defects in Children: Case–Control Study |
| title_full | Maternal Folic Acid Intake and Methylation Status of Genes Associated with Ventricular Septal Defects in Children: Case–Control Study |
| title_fullStr | Maternal Folic Acid Intake and Methylation Status of Genes Associated with Ventricular Septal Defects in Children: Case–Control Study |
| title_full_unstemmed | Maternal Folic Acid Intake and Methylation Status of Genes Associated with Ventricular Septal Defects in Children: Case–Control Study |
| title_short | Maternal Folic Acid Intake and Methylation Status of Genes Associated with Ventricular Septal Defects in Children: Case–Control Study |
| title_sort | maternal folic acid intake and methylation status of genes associated with ventricular septal defects in children case control study |
| topic | folic acid intake ventricular septal defects congenital heart disease methylation status <i>AXIN1</i> <i>MTHFR</i> |
| url | https://www.mdpi.com/2072-6643/13/6/2071 |
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