A protocol for mimicking lipid-mediated phase separation on the membrane using giant unilamellar vesicles

A protocol for mimicking lipid-mediated phase separation on the membrane using giant unilamellar vesicles

Summary: Here, we present a general protocol for mimicking lipid-mediated phase separation on the membrane using giant unilamellar vesicles (GUVs). In this protocol, we use GUVs to mimic Ago1 protein’s phase separation behavior on the membrane through binding with phosphoinositides (PIPs). We provid...

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Bibliographic Details
Main Authors: Yuanxiang Zhu, Yajie Gao, Qinmiao Sun, Dahua Chen
Format: Article
Language:English
Published: Elsevier 2022-12-01
Series:STAR Protocols
Subjects:
Cell biology
Cell membrane
Microscopy
Molecular biology
Protein biochemistry
Protein expression and purification
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166722005895

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http://www.sciencedirect.com/science/article/pii/S2666166722005895

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