Changes in Quality and Collagen Properties of Cattle Rumen Smooth Muscle Subjected to Repeated Freeze—Thaw Cycles
This study revealed changes in the quality, structural and functional collagen properties of cattle rumen smooth muscle (CSM) during F-T cycles. The results showed that thawing loss, pressing loss, β-galactosidase, β-glucuronidase activity, β-sheet content, emulsifying activity index (EAI), emulsion...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-10-01
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| Series: | Foods |
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| Online Access: | https://www.mdpi.com/2304-8158/11/21/3338 |
| _version_ | 1797468333909475328 |
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| author | Yinjuan Cao Zhaoyang Song Ling Han Qunli Yu Xiangying Kong Shibao Li |
| author_facet | Yinjuan Cao Zhaoyang Song Ling Han Qunli Yu Xiangying Kong Shibao Li |
| author_sort | Yinjuan Cao |
| collection | DOAJ |
| description | This study revealed changes in the quality, structural and functional collagen properties of cattle rumen smooth muscle (CSM) during F-T cycles. The results showed that thawing loss, pressing loss, β-galactosidase, β-glucuronidase activity, β-sheet content, emulsifying activity index (EAI), emulsion stability index (ESI), surface hydrophobicity, and turbidity of samples were significantly (<i>p</i> < 0.05) increased by 108.12%, 78.33%, 66.57%, 76.60%, 118.63%, 119.57%, 57.37%, 99.14%, and 82.35%, respectively, with increasing F-T cycles. Meanwhile, the shear force, pH, collagen content, α-helix content, thermal denaturation temperature (T<sub>max</sub>), and enthalpy value were significantly (<i>p</i> < 0.05) decreased by 30.88%, 3.19%, 33.23%, 35.92%, 10.34% and 46.51%, respectively. Scanning electron microscopy (SEM) and SDS-PAGE results indicated that F-T cycles induced an increase in disruption of CSM muscle microstructure and degradation of collagen. Thus, repeated F-T cycles promoted collagen degradation and structural disorder in CSM, while reducing the quality of CSM, but improving the functional collagen properties of CSM. These findings provide new data support for the development, processing, and quality control of CSM. |
| first_indexed | 2024-03-09T19:05:57Z |
| format | Article |
| id | doaj.art-f207a8e02f754f49a32f4db6a88d70f3 |
| institution | Directory Open Access Journal |
| issn | 2304-8158 |
| language | English |
| last_indexed | 2024-03-09T19:05:57Z |
| publishDate | 2022-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Foods |
| spelling | doaj.art-f207a8e02f754f49a32f4db6a88d70f32023-11-24T04:39:12ZengMDPI AGFoods2304-81582022-10-011121333810.3390/foods11213338Changes in Quality and Collagen Properties of Cattle Rumen Smooth Muscle Subjected to Repeated Freeze—Thaw CyclesYinjuan Cao0Zhaoyang Song1Ling Han2Qunli Yu3Xiangying Kong4Shibao Li5College of Food Science and Engineering, Gansu Agricultural University, Lanzhou 733070, ChinaCollege of Food Science and Engineering, Gansu Agricultural University, Lanzhou 733070, ChinaCollege of Food Science and Engineering, Gansu Agricultural University, Lanzhou 733070, ChinaCollege of Food Science and Engineering, Gansu Agricultural University, Lanzhou 733070, ChinaHaibei State Institute of Animal Husbandry and Veterinary Science, Haibei 812299, ChinaQinghai Cocosili Biological Engineering Co., Ltd., Xining 810100, ChinaThis study revealed changes in the quality, structural and functional collagen properties of cattle rumen smooth muscle (CSM) during F-T cycles. The results showed that thawing loss, pressing loss, β-galactosidase, β-glucuronidase activity, β-sheet content, emulsifying activity index (EAI), emulsion stability index (ESI), surface hydrophobicity, and turbidity of samples were significantly (<i>p</i> < 0.05) increased by 108.12%, 78.33%, 66.57%, 76.60%, 118.63%, 119.57%, 57.37%, 99.14%, and 82.35%, respectively, with increasing F-T cycles. Meanwhile, the shear force, pH, collagen content, α-helix content, thermal denaturation temperature (T<sub>max</sub>), and enthalpy value were significantly (<i>p</i> < 0.05) decreased by 30.88%, 3.19%, 33.23%, 35.92%, 10.34% and 46.51%, respectively. Scanning electron microscopy (SEM) and SDS-PAGE results indicated that F-T cycles induced an increase in disruption of CSM muscle microstructure and degradation of collagen. Thus, repeated F-T cycles promoted collagen degradation and structural disorder in CSM, while reducing the quality of CSM, but improving the functional collagen properties of CSM. These findings provide new data support for the development, processing, and quality control of CSM.https://www.mdpi.com/2304-8158/11/21/3338cattle rumen smooth musclefreeze-thawcollagenstructural and functional propertiesquality |
| spellingShingle | Yinjuan Cao Zhaoyang Song Ling Han Qunli Yu Xiangying Kong Shibao Li Changes in Quality and Collagen Properties of Cattle Rumen Smooth Muscle Subjected to Repeated Freeze—Thaw Cycles Foods cattle rumen smooth muscle freeze-thaw collagen structural and functional properties quality |
| title | Changes in Quality and Collagen Properties of Cattle Rumen Smooth Muscle Subjected to Repeated Freeze—Thaw Cycles |
| title_full | Changes in Quality and Collagen Properties of Cattle Rumen Smooth Muscle Subjected to Repeated Freeze—Thaw Cycles |
| title_fullStr | Changes in Quality and Collagen Properties of Cattle Rumen Smooth Muscle Subjected to Repeated Freeze—Thaw Cycles |
| title_full_unstemmed | Changes in Quality and Collagen Properties of Cattle Rumen Smooth Muscle Subjected to Repeated Freeze—Thaw Cycles |
| title_short | Changes in Quality and Collagen Properties of Cattle Rumen Smooth Muscle Subjected to Repeated Freeze—Thaw Cycles |
| title_sort | changes in quality and collagen properties of cattle rumen smooth muscle subjected to repeated freeze thaw cycles |
| topic | cattle rumen smooth muscle freeze-thaw collagen structural and functional properties quality |
| url | https://www.mdpi.com/2304-8158/11/21/3338 |
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