Optimized procedures for generating an enhanced, near physiological 2D culture system from porcine intestinal organoids

An important practical limitation of the three-dimensional geometry of stem-cell derived intestinal organoids is that it prevents easy access to the apical epithelium for testing food components, microorganisms, bioactive and toxic compounds. To this end, we here report on a new robust method for ge...

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Bibliographic Details
Main Authors: B. van der Hee, L.M.P. Loonen, N. Taverne, J.J. Taverne-Thiele, H. Smidt, J.M. Wells
Format: Article
Language:English
Published: Elsevier 2018-04-01
Series:Stem Cell Research
Online Access:http://www.sciencedirect.com/science/article/pii/S1873506118300576