An optimized isolation protocol yields high‐quality RNA from cassava tissues (Manihot esculenta Crantz)

An optimized isolation protocol yields high‐quality RNA from cassava tissues (Manihot esculenta Crantz)

We developed and modified a precise, rapid, and reproducible protocol isolating high‐quality RNA from tissues of multiple varieties of cassava plants (Manihot esculenta Crantz). The resulting method is suitable for use in mini, midi, and maxi preparations and rapidly achieves high total RNA yields (...

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Bibliographic Details
Main Authors: Babak Behnam, Adriana Bohorquez‐Chaux, Oscar Fernando Castaneda‐Mendez, Hiroyuki Tsuji, Manabu Ishitani, Luis Augusto Becerra Lopez‐Lavalle
Format: Article
Language:English
Published: Wiley 2019-04-01
Series:FEBS Open Bio
Subjects:
cassava
qRT‐PCR
RNA isolation
RNA sequencing
Online Access:https://doi.org/10.1002/2211-5463.12561

Internet

https://doi.org/10.1002/2211-5463.12561

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