Continuous fluorescence-based method for assessing Dicer cleavage efficiency reveals 3′ overhang nucleotide preference
The cleavage of double-stranded RNA (dsRNA) molecules by Dicer is a critical step in silencing genes by the RNA induced silencing complex (RISC). The development of Dicer substrates as nucleic acid–based therapeutics brings about a need to rapidly evaluate chemically modified RNA molecules for their...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
2010-04-01
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| Series: | BioTechniques |
| Subjects: | |
| Online Access: | https://www.future-science.com/doi/10.2144/000113402 |