CRISPR/Cas9-engineering of HMC-1.2 cells renders a human mast cell line with a single D816V-KIT mutation: An improved preclinical model for research on mastocytosis

CRISPR/Cas9-engineering of HMC-1.2 cells renders a human mast cell line with a single D816V-KIT mutation: An improved preclinical model for research on mastocytosis

The HMC-1.2 human mast cell (huMC) line is often employed in the study of attributes of neoplastic huMCs as found in patients with mastocytosis and their sensitivity to interventional drugs in vitro and in vivo. HMC-1.2 cells express constitutively active KIT, an essential growth factor receptor for...

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Bibliographic Details
Main Authors: Geethani Bandara, Guido H. Falduto, Andrea Luker, Yun Bai, Annika Pfeiffer, Justin Lack, Dean D. Metcalfe, Ana Olivera
Format: Article
Language:English
Published: Frontiers Media S.A. 2023-03-01
Series:Frontiers in Immunology
Subjects:
KIT variants
D816V-KIT
neoplastic human mast cells
mastocytosis
mast cell survival
HMC-1.2 cells
Online Access:https://www.frontiersin.org/articles/10.3389/fimmu.2023.1078958/full

Internet

https://www.frontiersin.org/articles/10.3389/fimmu.2023.1078958/full

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