Angiotensin-I Converting Enzyme Inhibition and Antioxidant Activity of Papain-Hydrolyzed Camel Whey Protein and Its Hepato-Renal Protective Effects in Thioacetamide-Induced Toxicity
Papain hydrolysis of camel whey protein (CWP) produced CWP hydrolysate (CWPH). Fractionation of CWPH by the size exclusion chromatography (SEC) generated fractions (i.e., SEC-F1 and SEC-F2). The angiotensin converting enzyme inhibitory activity (ACE-IA) and free radical scavenging actions were asses...
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MDPI AG
2021-02-01
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| Online Access: | https://www.mdpi.com/2304-8158/10/2/468 |
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| author | Ali Osman Abdalla El-Hadary Aida A. Korish Haifa M. AlNafea Manan A. Alhakbany Awad A. Awad Mahmoud Abdel-Hamid |
| author_facet | Ali Osman Abdalla El-Hadary Aida A. Korish Haifa M. AlNafea Manan A. Alhakbany Awad A. Awad Mahmoud Abdel-Hamid |
| author_sort | Ali Osman |
| collection | DOAJ |
| description | Papain hydrolysis of camel whey protein (CWP) produced CWP hydrolysate (CWPH). Fractionation of CWPH by the size exclusion chromatography (SEC) generated fractions (i.e., SEC-F1 and SEC-F2). The angiotensin converting enzyme inhibitory activity (ACE-IA) and free radical scavenging actions were assessed for CWP, CWPH, SEC-F1, and SEC-F2. The SEC-F2 exerted the highest ACE-IA and scavenging activities, followed by CWPH. The protective effects of CWPH on thioacetamide (TAA)-induced toxicity were investigated in rats. The liver enzymes, protein profile, lipid profile, antioxidant enzyme activities, renal functions, and liver histopathological changes were assessed. Animals with TAA toxicity showed impaired hepatorenal functions, hyperlipidemia, and decreased antioxidant capacity. Treatment by CWPH counteracted the TAA-induced oxidative tissue damage as well as preserved the renal and liver functions, the antioxidative enzyme activities, and the lipid profile, compared to the untreated animals. The current findings demonstrate that the ACE-IA and antioxidative effects of CWPH and its SEC-F2 fraction are worth noting. In addition, the CWPH antioxidative properties counteracted the toxic hepatorenal dysfunctions. It is concluded that the hydrolysis of CWP generates a wide range of bioactive peptides with potent antihypertensive, antioxidant, and hepatorenal protective properties. This opens up new prospects for the therapeutic utilization of CWPH and its fractions in the treatment of oxidative stress-associated health problems, e.g., hypertension and hepatorenal failure. |
| first_indexed | 2024-03-09T00:42:13Z |
| format | Article |
| id | doaj.art-f22976afefb74434b729fe6bbd4ae889 |
| institution | Directory Open Access Journal |
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| language | English |
| last_indexed | 2024-03-09T00:42:13Z |
| publishDate | 2021-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Foods |
| spelling | doaj.art-f22976afefb74434b729fe6bbd4ae8892023-12-11T17:47:17ZengMDPI AGFoods2304-81582021-02-0110246810.3390/foods10020468Angiotensin-I Converting Enzyme Inhibition and Antioxidant Activity of Papain-Hydrolyzed Camel Whey Protein and Its Hepato-Renal Protective Effects in Thioacetamide-Induced ToxicityAli Osman0Abdalla El-Hadary1Aida A. Korish2Haifa M. AlNafea3Manan A. Alhakbany4Awad A. Awad5Mahmoud Abdel-Hamid6Biochemistry Department, Faculty of Agriculture, Zagazig University, Zagazig 44511, EgyptBiochemistry Department, Faculty of Agriculture, Benha University, Benha 13736, EgyptPhysiology Department, College of Medicine, King Saud University Medical City, King Saud University, P.O. Box 2925, Riyadh 11461, Saudi ArabiaClinical Laboratory Sciences Department, College of Applied Medical Sciences, King Saud University, Riyadh 12284, Saudi ArabiaPhysiology Department, College of Medicine, King Saud University Medical City, King Saud University, P.O. Box 2925, Riyadh 11461, Saudi ArabiaDairy Science Department, Faculty of Agriculture, Cairo University, Giza 12613, EgyptDairy Science Department, Faculty of Agriculture, Cairo University, Giza 12613, EgyptPapain hydrolysis of camel whey protein (CWP) produced CWP hydrolysate (CWPH). Fractionation of CWPH by the size exclusion chromatography (SEC) generated fractions (i.e., SEC-F1 and SEC-F2). The angiotensin converting enzyme inhibitory activity (ACE-IA) and free radical scavenging actions were assessed for CWP, CWPH, SEC-F1, and SEC-F2. The SEC-F2 exerted the highest ACE-IA and scavenging activities, followed by CWPH. The protective effects of CWPH on thioacetamide (TAA)-induced toxicity were investigated in rats. The liver enzymes, protein profile, lipid profile, antioxidant enzyme activities, renal functions, and liver histopathological changes were assessed. Animals with TAA toxicity showed impaired hepatorenal functions, hyperlipidemia, and decreased antioxidant capacity. Treatment by CWPH counteracted the TAA-induced oxidative tissue damage as well as preserved the renal and liver functions, the antioxidative enzyme activities, and the lipid profile, compared to the untreated animals. The current findings demonstrate that the ACE-IA and antioxidative effects of CWPH and its SEC-F2 fraction are worth noting. In addition, the CWPH antioxidative properties counteracted the toxic hepatorenal dysfunctions. It is concluded that the hydrolysis of CWP generates a wide range of bioactive peptides with potent antihypertensive, antioxidant, and hepatorenal protective properties. This opens up new prospects for the therapeutic utilization of CWPH and its fractions in the treatment of oxidative stress-associated health problems, e.g., hypertension and hepatorenal failure.https://www.mdpi.com/2304-8158/10/2/468camel milk whey proteinpapainACE-inhibitory activityantioxidant activityhepatoprotective effectthioacetamide |
| spellingShingle | Ali Osman Abdalla El-Hadary Aida A. Korish Haifa M. AlNafea Manan A. Alhakbany Awad A. Awad Mahmoud Abdel-Hamid Angiotensin-I Converting Enzyme Inhibition and Antioxidant Activity of Papain-Hydrolyzed Camel Whey Protein and Its Hepato-Renal Protective Effects in Thioacetamide-Induced Toxicity Foods camel milk whey protein papain ACE-inhibitory activity antioxidant activity hepatoprotective effect thioacetamide |
| title | Angiotensin-I Converting Enzyme Inhibition and Antioxidant Activity of Papain-Hydrolyzed Camel Whey Protein and Its Hepato-Renal Protective Effects in Thioacetamide-Induced Toxicity |
| title_full | Angiotensin-I Converting Enzyme Inhibition and Antioxidant Activity of Papain-Hydrolyzed Camel Whey Protein and Its Hepato-Renal Protective Effects in Thioacetamide-Induced Toxicity |
| title_fullStr | Angiotensin-I Converting Enzyme Inhibition and Antioxidant Activity of Papain-Hydrolyzed Camel Whey Protein and Its Hepato-Renal Protective Effects in Thioacetamide-Induced Toxicity |
| title_full_unstemmed | Angiotensin-I Converting Enzyme Inhibition and Antioxidant Activity of Papain-Hydrolyzed Camel Whey Protein and Its Hepato-Renal Protective Effects in Thioacetamide-Induced Toxicity |
| title_short | Angiotensin-I Converting Enzyme Inhibition and Antioxidant Activity of Papain-Hydrolyzed Camel Whey Protein and Its Hepato-Renal Protective Effects in Thioacetamide-Induced Toxicity |
| title_sort | angiotensin i converting enzyme inhibition and antioxidant activity of papain hydrolyzed camel whey protein and its hepato renal protective effects in thioacetamide induced toxicity |
| topic | camel milk whey protein papain ACE-inhibitory activity antioxidant activity hepatoprotective effect thioacetamide |
| url | https://www.mdpi.com/2304-8158/10/2/468 |
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