Characterization of the broad-spectrum antibacterial activity of bacteriocin-like inhibitory substance-producing probiotics isolated from fermented foods
Abstract Antimicrobial peptides, such as bacteriocin, produced by probiotics have become a promising novel class of therapeutic agents for treating infectious diseases. Selected lactic acid bacteria (LAB) isolated from fermented foods with probiotic potential were evaluated for various tests, includ...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2024-03-01
|
| Series: | BMC Microbiology |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12866-024-03245-0 |
| _version_ | 1797259539266928640 |
|---|---|
| author | Tran Thi Dieu Thuy Hsu-Feng Lu Carl Jay Ballena Bregente Fong-Chi Annabelle Huang Pei-Chun Tu Cheng-Yen Kao |
| author_facet | Tran Thi Dieu Thuy Hsu-Feng Lu Carl Jay Ballena Bregente Fong-Chi Annabelle Huang Pei-Chun Tu Cheng-Yen Kao |
| author_sort | Tran Thi Dieu Thuy |
| collection | DOAJ |
| description | Abstract Antimicrobial peptides, such as bacteriocin, produced by probiotics have become a promising novel class of therapeutic agents for treating infectious diseases. Selected lactic acid bacteria (LAB) isolated from fermented foods with probiotic potential were evaluated for various tests, including exopolysaccharide production, antibiotic susceptibility, acid and bile tolerance, antibacterial activity, and cell adhesion and cytotoxicity to gastric cell lines. Six selected LAB strains maintained their high viability under gastrointestinal conditions, produced high exopolysaccharides, showed no or less cytotoxicity, and adhered successfully to gastric cells. Furthermore, three strains, Weissella confusa CYLB30, Lactiplantibacillus plantarum CYLB47, and Limosilactobacillus fermentum CYLB55, demonstrated a strong antibacterial effect against drug-resistant Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, Salmonella enterica serovar Choleraesuis, Enterococcus faecium, and Staphylococcus aureus. Whole genome sequencing was performed on these three strains using the Nanopore platform; then, the results showed that all three strains did not harbor genes related to toxins, superantigens, and acquired antimicrobial resistance, in their genome. The bacteriocin gene cluster was found in CYLB47 genome, but not in CYLB30 and CYLB55 genomes. In SDS-PAGE, the extract of CYLB30 and CYLB47 bacteriocin-like inhibitory substance (BLIS) yielded a single band with a size of less than 10 kDa. These BLIS inhibited the growth and biofilm formation of drug-resistant P. aeruginosa and methicillin-resistant S. aureus (MRSA), causing membrane disruption and inhibiting adhesion ability to human skin HaCaT cells. Moreover, CYLB30 and CYLB47 BLIS rescued the larvae after being infected with P. aeruginosa and MRSA infections. In conclusion, CYLB30 and CYLB47 BLIS may be potential alternative treatment for multidrug-resistant bacteria infections. |
| first_indexed | 2024-04-24T23:11:02Z |
| format | Article |
| id | doaj.art-f25fbdd599ef4f4c9005bd27aef88c68 |
| institution | Directory Open Access Journal |
| issn | 1471-2180 |
| language | English |
| last_indexed | 2024-04-24T23:11:02Z |
| publishDate | 2024-03-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Microbiology |
| spelling | doaj.art-f25fbdd599ef4f4c9005bd27aef88c682024-03-17T12:17:19ZengBMCBMC Microbiology1471-21802024-03-0124111510.1186/s12866-024-03245-0Characterization of the broad-spectrum antibacterial activity of bacteriocin-like inhibitory substance-producing probiotics isolated from fermented foodsTran Thi Dieu Thuy0Hsu-Feng Lu1Carl Jay Ballena Bregente2Fong-Chi Annabelle Huang3Pei-Chun Tu4Cheng-Yen Kao5Institute of Microbiology and Immunology, College of Life Sciences, National Yang Ming Chiao Tung UniversityDepartment of Laboratory Medicine, China Medical University HospitalInstitute of Microbiology and Immunology, College of Life Sciences, National Yang Ming Chiao Tung UniversityTaipei American SchoolInstitute of Microbiology and Immunology, College of Life Sciences, National Yang Ming Chiao Tung UniversityInstitute of Microbiology and Immunology, College of Life Sciences, National Yang Ming Chiao Tung UniversityAbstract Antimicrobial peptides, such as bacteriocin, produced by probiotics have become a promising novel class of therapeutic agents for treating infectious diseases. Selected lactic acid bacteria (LAB) isolated from fermented foods with probiotic potential were evaluated for various tests, including exopolysaccharide production, antibiotic susceptibility, acid and bile tolerance, antibacterial activity, and cell adhesion and cytotoxicity to gastric cell lines. Six selected LAB strains maintained their high viability under gastrointestinal conditions, produced high exopolysaccharides, showed no or less cytotoxicity, and adhered successfully to gastric cells. Furthermore, three strains, Weissella confusa CYLB30, Lactiplantibacillus plantarum CYLB47, and Limosilactobacillus fermentum CYLB55, demonstrated a strong antibacterial effect against drug-resistant Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, Salmonella enterica serovar Choleraesuis, Enterococcus faecium, and Staphylococcus aureus. Whole genome sequencing was performed on these three strains using the Nanopore platform; then, the results showed that all three strains did not harbor genes related to toxins, superantigens, and acquired antimicrobial resistance, in their genome. The bacteriocin gene cluster was found in CYLB47 genome, but not in CYLB30 and CYLB55 genomes. In SDS-PAGE, the extract of CYLB30 and CYLB47 bacteriocin-like inhibitory substance (BLIS) yielded a single band with a size of less than 10 kDa. These BLIS inhibited the growth and biofilm formation of drug-resistant P. aeruginosa and methicillin-resistant S. aureus (MRSA), causing membrane disruption and inhibiting adhesion ability to human skin HaCaT cells. Moreover, CYLB30 and CYLB47 BLIS rescued the larvae after being infected with P. aeruginosa and MRSA infections. In conclusion, CYLB30 and CYLB47 BLIS may be potential alternative treatment for multidrug-resistant bacteria infections.https://doi.org/10.1186/s12866-024-03245-0Antibacterial activityBacteriocin-like inhibitory substanceBiofilm formationMultidrug-resistant bacteriaProbiotic |
| spellingShingle | Tran Thi Dieu Thuy Hsu-Feng Lu Carl Jay Ballena Bregente Fong-Chi Annabelle Huang Pei-Chun Tu Cheng-Yen Kao Characterization of the broad-spectrum antibacterial activity of bacteriocin-like inhibitory substance-producing probiotics isolated from fermented foods BMC Microbiology Antibacterial activity Bacteriocin-like inhibitory substance Biofilm formation Multidrug-resistant bacteria Probiotic |
| title | Characterization of the broad-spectrum antibacterial activity of bacteriocin-like inhibitory substance-producing probiotics isolated from fermented foods |
| title_full | Characterization of the broad-spectrum antibacterial activity of bacteriocin-like inhibitory substance-producing probiotics isolated from fermented foods |
| title_fullStr | Characterization of the broad-spectrum antibacterial activity of bacteriocin-like inhibitory substance-producing probiotics isolated from fermented foods |
| title_full_unstemmed | Characterization of the broad-spectrum antibacterial activity of bacteriocin-like inhibitory substance-producing probiotics isolated from fermented foods |
| title_short | Characterization of the broad-spectrum antibacterial activity of bacteriocin-like inhibitory substance-producing probiotics isolated from fermented foods |
| title_sort | characterization of the broad spectrum antibacterial activity of bacteriocin like inhibitory substance producing probiotics isolated from fermented foods |
| topic | Antibacterial activity Bacteriocin-like inhibitory substance Biofilm formation Multidrug-resistant bacteria Probiotic |
| url | https://doi.org/10.1186/s12866-024-03245-0 |
| work_keys_str_mv | AT tranthidieuthuy characterizationofthebroadspectrumantibacterialactivityofbacteriocinlikeinhibitorysubstanceproducingprobioticsisolatedfromfermentedfoods AT hsufenglu characterizationofthebroadspectrumantibacterialactivityofbacteriocinlikeinhibitorysubstanceproducingprobioticsisolatedfromfermentedfoods AT carljayballenabregente characterizationofthebroadspectrumantibacterialactivityofbacteriocinlikeinhibitorysubstanceproducingprobioticsisolatedfromfermentedfoods AT fongchiannabellehuang characterizationofthebroadspectrumantibacterialactivityofbacteriocinlikeinhibitorysubstanceproducingprobioticsisolatedfromfermentedfoods AT peichuntu characterizationofthebroadspectrumantibacterialactivityofbacteriocinlikeinhibitorysubstanceproducingprobioticsisolatedfromfermentedfoods AT chengyenkao characterizationofthebroadspectrumantibacterialactivityofbacteriocinlikeinhibitorysubstanceproducingprobioticsisolatedfromfermentedfoods |