Fluorescent sensors reporting the activity of ammonium transceptors in live cells

Fluorescent sensors reporting the activity of ammonium transceptors in live cells

Ammonium serves as key nitrogen source and metabolic intermediate, yet excess causes toxicity. Ammonium uptake is mediated by ammonium transporters, whose regulation is poorly understood. While transport can easily be characterized in heterologous systems, measuring transporter activity in vivo rema...

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Bibliographic Details
Main Authors: Roberto De Michele, Cindy Ast, Dominique Loqué, Cheng-Hsun Ho, Susana LA Andrade, Viviane Lanquar, Guido Grossmann, Sören Gehne, Michael U Kumke, Wolf B Frommer
Format: Article
Language:English
Published: eLife Sciences Publications Ltd 2013-07-01
Series:eLife
Subjects:
transport
conformation
biosensor
ammonium
GFP
fluorescent probe
Online Access:https://elifesciences.org/articles/00800

Internet

https://elifesciences.org/articles/00800

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