Antigen retrieval by citrate solution improves western blot signal

In the present work, we describe and evaluate an additional step to the standard western blot protocol to increase signal strength after revealing. Weak or absence of signal is a common issue in western blot protocol leading to unexpected results. In our Antigen Retrieval for Western Blot Method (AR...

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Bibliographic Details
Main Authors: Daniel Patiño-García, Nadia Rocha-Pérez, Ricardo D. Moreno, Renan Orellana
Format: Article
Language:English
Published: Elsevier 2019-01-01
Series:MethodsX
Online Access:http://www.sciencedirect.com/science/article/pii/S221501611930041X