Improved effect of a mitochondria-targeted antioxidant on hydrogen peroxide-induced oxidative stress in human retinal pigment epithelium cells
Abstract Background Oxidative damage to retinal pigment epithelial (RPE) cells contributes to the development of age-related macular degeneration, which is among the leading causes of visual loss in elderly people. In the present study, we evaluated the protective role of triphenylphosphonium (TPP)-...
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BMC
2021-01-01
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Series: | BMC Pharmacology and Toxicology |
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Online Access: | https://doi.org/10.1186/s40360-020-00471-w |
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author | Myung Hee Kim Do-Hun Kim Su Geun Yang Dae Yu Kim |
author_facet | Myung Hee Kim Do-Hun Kim Su Geun Yang Dae Yu Kim |
author_sort | Myung Hee Kim |
collection | DOAJ |
description | Abstract Background Oxidative damage to retinal pigment epithelial (RPE) cells contributes to the development of age-related macular degeneration, which is among the leading causes of visual loss in elderly people. In the present study, we evaluated the protective role of triphenylphosphonium (TPP)-Niacin against hydrogen peroxide (H2O2)-induced oxidative stress in RPE cells. Methods The cellular viability, lactate dehydrogenase release, reactive oxygen species (ROS) generation, and mitochondrial function of retinal ARPE-19 cells were determined under treatment with H2O2 or pre-treatment with TPP-Niacin. The expression level of mitochondrial related genes and some transcription factors were assessed using real-time polymerase chain reaction (RT-qPCR). Results TPP-Niacin significantly improved cell viability, reduced ROS generation, and increased the antioxidant enzymes in H2O2-treated ARPE-19 cells. Mitochondrial dysfunction from the H2O2-induced oxidative stress was also considerably diminished by TPP-Niacin treatment, along with reduction of the mitochondrial membrane potential (MMP) and upregulation of the mitochondrial-associated gene. In addition, TPP-Niacin markedly enhanced the expression of transcription factors (PGC-1α and NRF2) and antioxidant-associated genes (especially HO-1 and NQO-1). Conclusion We verified the protective effect of TPP-Niacin against H2O2-induced oxidative stress in RPE cells. TPP-Niacin is believed to protect against mitochondrial dysfunction by upregulating antioxidant-related genes, such as PGC-1α, NRF2, HO-1, and NQO-1, in RPE cells. |
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institution | Directory Open Access Journal |
issn | 2050-6511 |
language | English |
last_indexed | 2024-12-17T20:18:21Z |
publishDate | 2021-01-01 |
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series | BMC Pharmacology and Toxicology |
spelling | doaj.art-f2afcd3101fb421e96dcde6b80c22d982022-12-21T21:34:01ZengBMCBMC Pharmacology and Toxicology2050-65112021-01-0122111310.1186/s40360-020-00471-wImproved effect of a mitochondria-targeted antioxidant on hydrogen peroxide-induced oxidative stress in human retinal pigment epithelium cellsMyung Hee Kim0Do-Hun Kim1Su Geun Yang2Dae Yu Kim3Inha Research Institute for Aerospace Medicine, Inha UniversityInha Research Institute for Aerospace Medicine, Inha UniversityInha Research Institute for Aerospace Medicine, Inha UniversityInha Research Institute for Aerospace Medicine, Inha UniversityAbstract Background Oxidative damage to retinal pigment epithelial (RPE) cells contributes to the development of age-related macular degeneration, which is among the leading causes of visual loss in elderly people. In the present study, we evaluated the protective role of triphenylphosphonium (TPP)-Niacin against hydrogen peroxide (H2O2)-induced oxidative stress in RPE cells. Methods The cellular viability, lactate dehydrogenase release, reactive oxygen species (ROS) generation, and mitochondrial function of retinal ARPE-19 cells were determined under treatment with H2O2 or pre-treatment with TPP-Niacin. The expression level of mitochondrial related genes and some transcription factors were assessed using real-time polymerase chain reaction (RT-qPCR). Results TPP-Niacin significantly improved cell viability, reduced ROS generation, and increased the antioxidant enzymes in H2O2-treated ARPE-19 cells. Mitochondrial dysfunction from the H2O2-induced oxidative stress was also considerably diminished by TPP-Niacin treatment, along with reduction of the mitochondrial membrane potential (MMP) and upregulation of the mitochondrial-associated gene. In addition, TPP-Niacin markedly enhanced the expression of transcription factors (PGC-1α and NRF2) and antioxidant-associated genes (especially HO-1 and NQO-1). Conclusion We verified the protective effect of TPP-Niacin against H2O2-induced oxidative stress in RPE cells. TPP-Niacin is believed to protect against mitochondrial dysfunction by upregulating antioxidant-related genes, such as PGC-1α, NRF2, HO-1, and NQO-1, in RPE cells.https://doi.org/10.1186/s40360-020-00471-wAge-related macular degenerationRetinal pigment epitheliumMitochondrial functionAntioxidants |
spellingShingle | Myung Hee Kim Do-Hun Kim Su Geun Yang Dae Yu Kim Improved effect of a mitochondria-targeted antioxidant on hydrogen peroxide-induced oxidative stress in human retinal pigment epithelium cells BMC Pharmacology and Toxicology Age-related macular degeneration Retinal pigment epithelium Mitochondrial function Antioxidants |
title | Improved effect of a mitochondria-targeted antioxidant on hydrogen peroxide-induced oxidative stress in human retinal pigment epithelium cells |
title_full | Improved effect of a mitochondria-targeted antioxidant on hydrogen peroxide-induced oxidative stress in human retinal pigment epithelium cells |
title_fullStr | Improved effect of a mitochondria-targeted antioxidant on hydrogen peroxide-induced oxidative stress in human retinal pigment epithelium cells |
title_full_unstemmed | Improved effect of a mitochondria-targeted antioxidant on hydrogen peroxide-induced oxidative stress in human retinal pigment epithelium cells |
title_short | Improved effect of a mitochondria-targeted antioxidant on hydrogen peroxide-induced oxidative stress in human retinal pigment epithelium cells |
title_sort | improved effect of a mitochondria targeted antioxidant on hydrogen peroxide induced oxidative stress in human retinal pigment epithelium cells |
topic | Age-related macular degeneration Retinal pigment epithelium Mitochondrial function Antioxidants |
url | https://doi.org/10.1186/s40360-020-00471-w |
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