Improved effect of a mitochondria-targeted antioxidant on hydrogen peroxide-induced oxidative stress in human retinal pigment epithelium cells

Abstract Background Oxidative damage to retinal pigment epithelial (RPE) cells contributes to the development of age-related macular degeneration, which is among the leading causes of visual loss in elderly people. In the present study, we evaluated the protective role of triphenylphosphonium (TPP)-...

Full description

Bibliographic Details
Main Authors: Myung Hee Kim, Do-Hun Kim, Su Geun Yang, Dae Yu Kim
Format: Article
Language:English
Published: BMC 2021-01-01
Series:BMC Pharmacology and Toxicology
Subjects:
Online Access:https://doi.org/10.1186/s40360-020-00471-w
_version_ 1818720156427223040
author Myung Hee Kim
Do-Hun Kim
Su Geun Yang
Dae Yu Kim
author_facet Myung Hee Kim
Do-Hun Kim
Su Geun Yang
Dae Yu Kim
author_sort Myung Hee Kim
collection DOAJ
description Abstract Background Oxidative damage to retinal pigment epithelial (RPE) cells contributes to the development of age-related macular degeneration, which is among the leading causes of visual loss in elderly people. In the present study, we evaluated the protective role of triphenylphosphonium (TPP)-Niacin against hydrogen peroxide (H2O2)-induced oxidative stress in RPE cells. Methods The cellular viability, lactate dehydrogenase release, reactive oxygen species (ROS) generation, and mitochondrial function of retinal ARPE-19 cells were determined under treatment with H2O2 or pre-treatment with TPP-Niacin. The expression level of mitochondrial related genes and some transcription factors were assessed using real-time polymerase chain reaction (RT-qPCR). Results TPP-Niacin significantly improved cell viability, reduced ROS generation, and increased the antioxidant enzymes in H2O2-treated ARPE-19 cells. Mitochondrial dysfunction from the H2O2-induced oxidative stress was also considerably diminished by TPP-Niacin treatment, along with reduction of the mitochondrial membrane potential (MMP) and upregulation of the mitochondrial-associated gene. In addition, TPP-Niacin markedly enhanced the expression of transcription factors (PGC-1α and NRF2) and antioxidant-associated genes (especially HO-1 and NQO-1). Conclusion We verified the protective effect of TPP-Niacin against H2O2-induced oxidative stress in RPE cells. TPP-Niacin is believed to protect against mitochondrial dysfunction by upregulating antioxidant-related genes, such as PGC-1α, NRF2, HO-1, and NQO-1, in RPE cells.
first_indexed 2024-12-17T20:18:21Z
format Article
id doaj.art-f2afcd3101fb421e96dcde6b80c22d98
institution Directory Open Access Journal
issn 2050-6511
language English
last_indexed 2024-12-17T20:18:21Z
publishDate 2021-01-01
publisher BMC
record_format Article
series BMC Pharmacology and Toxicology
spelling doaj.art-f2afcd3101fb421e96dcde6b80c22d982022-12-21T21:34:01ZengBMCBMC Pharmacology and Toxicology2050-65112021-01-0122111310.1186/s40360-020-00471-wImproved effect of a mitochondria-targeted antioxidant on hydrogen peroxide-induced oxidative stress in human retinal pigment epithelium cellsMyung Hee Kim0Do-Hun Kim1Su Geun Yang2Dae Yu Kim3Inha Research Institute for Aerospace Medicine, Inha UniversityInha Research Institute for Aerospace Medicine, Inha UniversityInha Research Institute for Aerospace Medicine, Inha UniversityInha Research Institute for Aerospace Medicine, Inha UniversityAbstract Background Oxidative damage to retinal pigment epithelial (RPE) cells contributes to the development of age-related macular degeneration, which is among the leading causes of visual loss in elderly people. In the present study, we evaluated the protective role of triphenylphosphonium (TPP)-Niacin against hydrogen peroxide (H2O2)-induced oxidative stress in RPE cells. Methods The cellular viability, lactate dehydrogenase release, reactive oxygen species (ROS) generation, and mitochondrial function of retinal ARPE-19 cells were determined under treatment with H2O2 or pre-treatment with TPP-Niacin. The expression level of mitochondrial related genes and some transcription factors were assessed using real-time polymerase chain reaction (RT-qPCR). Results TPP-Niacin significantly improved cell viability, reduced ROS generation, and increased the antioxidant enzymes in H2O2-treated ARPE-19 cells. Mitochondrial dysfunction from the H2O2-induced oxidative stress was also considerably diminished by TPP-Niacin treatment, along with reduction of the mitochondrial membrane potential (MMP) and upregulation of the mitochondrial-associated gene. In addition, TPP-Niacin markedly enhanced the expression of transcription factors (PGC-1α and NRF2) and antioxidant-associated genes (especially HO-1 and NQO-1). Conclusion We verified the protective effect of TPP-Niacin against H2O2-induced oxidative stress in RPE cells. TPP-Niacin is believed to protect against mitochondrial dysfunction by upregulating antioxidant-related genes, such as PGC-1α, NRF2, HO-1, and NQO-1, in RPE cells.https://doi.org/10.1186/s40360-020-00471-wAge-related macular degenerationRetinal pigment epitheliumMitochondrial functionAntioxidants
spellingShingle Myung Hee Kim
Do-Hun Kim
Su Geun Yang
Dae Yu Kim
Improved effect of a mitochondria-targeted antioxidant on hydrogen peroxide-induced oxidative stress in human retinal pigment epithelium cells
BMC Pharmacology and Toxicology
Age-related macular degeneration
Retinal pigment epithelium
Mitochondrial function
Antioxidants
title Improved effect of a mitochondria-targeted antioxidant on hydrogen peroxide-induced oxidative stress in human retinal pigment epithelium cells
title_full Improved effect of a mitochondria-targeted antioxidant on hydrogen peroxide-induced oxidative stress in human retinal pigment epithelium cells
title_fullStr Improved effect of a mitochondria-targeted antioxidant on hydrogen peroxide-induced oxidative stress in human retinal pigment epithelium cells
title_full_unstemmed Improved effect of a mitochondria-targeted antioxidant on hydrogen peroxide-induced oxidative stress in human retinal pigment epithelium cells
title_short Improved effect of a mitochondria-targeted antioxidant on hydrogen peroxide-induced oxidative stress in human retinal pigment epithelium cells
title_sort improved effect of a mitochondria targeted antioxidant on hydrogen peroxide induced oxidative stress in human retinal pigment epithelium cells
topic Age-related macular degeneration
Retinal pigment epithelium
Mitochondrial function
Antioxidants
url https://doi.org/10.1186/s40360-020-00471-w
work_keys_str_mv AT myungheekim improvedeffectofamitochondriatargetedantioxidantonhydrogenperoxideinducedoxidativestressinhumanretinalpigmentepitheliumcells
AT dohunkim improvedeffectofamitochondriatargetedantioxidantonhydrogenperoxideinducedoxidativestressinhumanretinalpigmentepitheliumcells
AT sugeunyang improvedeffectofamitochondriatargetedantioxidantonhydrogenperoxideinducedoxidativestressinhumanretinalpigmentepitheliumcells
AT daeyukim improvedeffectofamitochondriatargetedantioxidantonhydrogenperoxideinducedoxidativestressinhumanretinalpigmentepitheliumcells