Enrichment of transgene integrations by transient CRISPR activation of a silent reporter gene

Enrichment of transgene integrations by transient CRISPR activation of a silent reporter gene

CRISPR-Cas-mediated site-specific integration of transgenes by homology-directed repair (HDR) is challenging, especially in primary cells, where inferior editing efficiency may impede the development of gene- and cellular therapies. Various strategies for enrichment of cells with transgene integrati...

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Bibliographic Details
Main Authors: Nanna S. Mikkelsen, Sabina S. Hernandez, Trine I. Jensen, Jessica L. Schneller, Rasmus O. Bak
Format: Article
Language:English
Published: Elsevier 2023-06-01
Series:Molecular Therapy: Methods & Clinical Development
Subjects:
transgene integration
enrichment
selection
CRISPR
reporter gene
CAR T
Online Access:http://www.sciencedirect.com/science/article/pii/S2329050123000207

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http://www.sciencedirect.com/science/article/pii/S2329050123000207

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