Establishment of Cysteine Detection Method in Food Based on Fluorescent Probe
A novel, simple and sensitive fluorescenct method for determination of cysteine in water, milk, milk powder, cabbage, apple, pear and radish was established based on rosamine fluorescent probe. The probe was constructed by rosamine as the signal reporter and phenylalkynyl as the reaction site. Throu...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | zho |
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The editorial department of Science and Technology of Food Industry
2022-02-01
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| Series: | Shipin gongye ke-ji |
| Subjects: | |
| Online Access: | http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2021060066 |
| _version_ | 1798017482420649984 |
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| author | Rui HAN Qiong WU Xin ZHAO Shuhong MAO |
| author_facet | Rui HAN Qiong WU Xin ZHAO Shuhong MAO |
| author_sort | Rui HAN |
| collection | DOAJ |
| description | A novel, simple and sensitive fluorescenct method for determination of cysteine in water, milk, milk powder, cabbage, apple, pear and radish was established based on rosamine fluorescent probe. The probe was constructed by rosamine as the signal reporter and phenylalkynyl as the reaction site. Through Michael addition and intramolecular reaction, the probe could detect cysteine with high selectivity and sensitivity. Results indicated that under the optimized conditions of phosphoric acid buffer concentration of 10 mmol/L, pH9.5, methanol/water volume ratio of 1:9 and room temperature reaction for 35 min, the probe selectively recognized cysteine, and its relative fluorescence intensity increased with the increasing of cysteine concentration. Furthermore, the fluorescence signal of the probe had a good linear relationship with cysteine in the concentration range from 18.0 to 70.0 μmol/L (R2=0.992) with detection limit of 0.18 μmol/L. Furthermore, the method was used to determinate cysteine in water, milk, milk powder, cabbage, apple, pear and radish with good recoveries (96.4%~102.97%) and RSD (0.1%～2%). This suggested that the approach would have high selectivity, sensitivity and the feasibility of cysteine detection in practical sample, as well as a good application prospect. |
| first_indexed | 2024-04-11T16:07:57Z |
| format | Article |
| id | doaj.art-f2d2f9fed5714ce4a1753359da801fa2 |
| institution | Directory Open Access Journal |
| issn | 1002-0306 |
| language | zho |
| last_indexed | 2024-04-11T16:07:57Z |
| publishDate | 2022-02-01 |
| publisher | The editorial department of Science and Technology of Food Industry |
| record_format | Article |
| series | Shipin gongye ke-ji |
| spelling | doaj.art-f2d2f9fed5714ce4a1753359da801fa22022-12-22T04:14:47ZzhoThe editorial department of Science and Technology of Food IndustryShipin gongye ke-ji1002-03062022-02-0143430531110.13386/j.issn1002-0306.20210600662021060066-4Establishment of Cysteine Detection Method in Food Based on Fluorescent ProbeRui HAN0Qiong WU1Xin ZHAO2Shuhong MAO3School of Biotechnology, Tianjin Univercity of Science & Technology, Tianjin 300457, ChinaSchool of Biotechnology, Tianjin Univercity of Science & Technology, Tianjin 300457, ChinaSchool of Biotechnology, Tianjin Univercity of Science & Technology, Tianjin 300457, ChinaSchool of Biotechnology, Tianjin Univercity of Science & Technology, Tianjin 300457, ChinaA novel, simple and sensitive fluorescenct method for determination of cysteine in water, milk, milk powder, cabbage, apple, pear and radish was established based on rosamine fluorescent probe. The probe was constructed by rosamine as the signal reporter and phenylalkynyl as the reaction site. Through Michael addition and intramolecular reaction, the probe could detect cysteine with high selectivity and sensitivity. Results indicated that under the optimized conditions of phosphoric acid buffer concentration of 10 mmol/L, pH9.5, methanol/water volume ratio of 1:9 and room temperature reaction for 35 min, the probe selectively recognized cysteine, and its relative fluorescence intensity increased with the increasing of cysteine concentration. Furthermore, the fluorescence signal of the probe had a good linear relationship with cysteine in the concentration range from 18.0 to 70.0 μmol/L (R2=0.992) with detection limit of 0.18 μmol/L. Furthermore, the method was used to determinate cysteine in water, milk, milk powder, cabbage, apple, pear and radish with good recoveries (96.4%~102.97%) and RSD (0.1%～2%). This suggested that the approach would have high selectivity, sensitivity and the feasibility of cysteine detection in practical sample, as well as a good application prospect.http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2021060066rosaminecysteinephenylacetylenefluorescent probemichael addition |
| spellingShingle | Rui HAN Qiong WU Xin ZHAO Shuhong MAO Establishment of Cysteine Detection Method in Food Based on Fluorescent Probe Shipin gongye ke-ji rosamine cysteine phenylacetylene fluorescent probe michael addition |
| title | Establishment of Cysteine Detection Method in Food Based on Fluorescent Probe |
| title_full | Establishment of Cysteine Detection Method in Food Based on Fluorescent Probe |
| title_fullStr | Establishment of Cysteine Detection Method in Food Based on Fluorescent Probe |
| title_full_unstemmed | Establishment of Cysteine Detection Method in Food Based on Fluorescent Probe |
| title_short | Establishment of Cysteine Detection Method in Food Based on Fluorescent Probe |
| title_sort | establishment of cysteine detection method in food based on fluorescent probe |
| topic | rosamine cysteine phenylacetylene fluorescent probe michael addition |
| url | http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2021060066 |
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