Fluorogenic in vitro activity assay for the main protease Mpro from SARS-CoV-2 and its adaptation to the identification of inhibitors

Fluorogenic in vitro activity assay for the main protease Mpro from SARS-CoV-2 and its adaptation to the identification of inhibitors

Summary: This protocol describes an in vitro fluorogenic assay to measure the proteolytic activity and identify inhibitors of Mpro, the main protease produced by SARS-CoV-2 (Severe acute respiratory syndrome coronavirus 2). Studies to identify potential inhibitors of Mpro mainly rely on in silico mo...

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Bibliographic Details
Main Authors: Julien Ihssen, Greta Faccio, Chunyan Yao, Teja Sirec, Urs Spitz
Format: Article
Language:English
Published: Elsevier 2021-09-01
Series:STAR Protocols
Subjects:
Biotechnology and bioengineering
Chemistry
Clinical Protocol
Health Sciences
High Throughput Screening
Molecular/Chemical Probes
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166721004998

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http://www.sciencedirect.com/science/article/pii/S2666166721004998

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