Retrofitting the BAC cloning vector pBeloBAC11 by the insertion of a mutant loxP site

Abstract Objective Human genomic libraries constructed in bacterial artificial chromosome vectors were utilized to make physical maps of all 23-chromosome pairs and as the templates for DNA sequencing to aid in the completion of the Human Genome Project. The goal of this study was to modify the BAC...

Full description

Bibliographic Details
Main Author: Jonathon S. Coren
Format: Article
Language:English
Published: BMC 2017-07-01
Series:BMC Research Notes
Subjects:
Online Access:http://link.springer.com/article/10.1186/s13104-017-2631-8