Retrofitting the BAC cloning vector pBeloBAC11 by the insertion of a mutant loxP site
Abstract Objective Human genomic libraries constructed in bacterial artificial chromosome vectors were utilized to make physical maps of all 23-chromosome pairs and as the templates for DNA sequencing to aid in the completion of the Human Genome Project. The goal of this study was to modify the BAC...
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Format: | Article |
Language: | English |
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BMC
2017-07-01
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Series: | BMC Research Notes |
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Online Access: | http://link.springer.com/article/10.1186/s13104-017-2631-8 |