A recombinant Modified Vaccinia virus Ankara expressing prME of tick-borne encephalitis virus affords mice full protection against TBEV infection
IntroductionTick-borne encephalitis virus (TBEV) is an important human pathogen that can cause a serious disease involving the central nervous system (tick-borne encephalitis, TBE). Although approved inactivated vaccines are available, the number of TBE cases is rising, and breakthrough infections i...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-04-01
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| Series: | Frontiers in Immunology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fimmu.2023.1182963/full |
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| author | Mareike Kubinski Jana Beicht Isabel Zdora Isabel Zdora Jeannine Biermann Christina Puff Thomas Gerlach Alina Tscherne Alina Tscherne Wolfgang Baumgärtner Wolfgang Baumgärtner Albert D. M. E. Osterhaus Gerd Sutter Gerd Sutter Chittappen Kandiyil Prajeeth Guus F. Rimmelzwaan |
| author_facet | Mareike Kubinski Jana Beicht Isabel Zdora Isabel Zdora Jeannine Biermann Christina Puff Thomas Gerlach Alina Tscherne Alina Tscherne Wolfgang Baumgärtner Wolfgang Baumgärtner Albert D. M. E. Osterhaus Gerd Sutter Gerd Sutter Chittappen Kandiyil Prajeeth Guus F. Rimmelzwaan |
| author_sort | Mareike Kubinski |
| collection | DOAJ |
| description | IntroductionTick-borne encephalitis virus (TBEV) is an important human pathogen that can cause a serious disease involving the central nervous system (tick-borne encephalitis, TBE). Although approved inactivated vaccines are available, the number of TBE cases is rising, and breakthrough infections in fully vaccinated subjects have been reported in recent years.MethodsIn the present study, we generated and characterized a recombinant Modified Vaccinia virus Ankara (MVA) for the delivery of the pre-membrane (prM) and envelope (E) proteins of TBEV (MVA-prME).ResultsMVA-prME was tested in mice in comparison with a licensed vaccine FSME-IMMUN® and proved to be highly immunogenic and afforded full protection against challenge infection with TBEV.DiscussionOur data indicate that MVA-prME holds promise as an improved next-generation vaccine for the prevention of TBE. |
| first_indexed | 2024-04-09T17:02:34Z |
| format | Article |
| id | doaj.art-f30976dabf954191adc669ce1ae55027 |
| institution | Directory Open Access Journal |
| issn | 1664-3224 |
| language | English |
| last_indexed | 2024-04-09T17:02:34Z |
| publishDate | 2023-04-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Immunology |
| spelling | doaj.art-f30976dabf954191adc669ce1ae550272023-04-21T04:33:59ZengFrontiers Media S.A.Frontiers in Immunology1664-32242023-04-011410.3389/fimmu.2023.11829631182963A recombinant Modified Vaccinia virus Ankara expressing prME of tick-borne encephalitis virus affords mice full protection against TBEV infectionMareike Kubinski0Jana Beicht1Isabel Zdora2Isabel Zdora3Jeannine Biermann4Christina Puff5Thomas Gerlach6Alina Tscherne7Alina Tscherne8Wolfgang Baumgärtner9Wolfgang Baumgärtner10Albert D. M. E. Osterhaus11Gerd Sutter12Gerd Sutter13Chittappen Kandiyil Prajeeth14Guus F. Rimmelzwaan15Research Center for Emerging Infections and Zoonoses, University of Veterinary Medicine Hannover, Foundation, Hannover, GermanyResearch Center for Emerging Infections and Zoonoses, University of Veterinary Medicine Hannover, Foundation, Hannover, GermanyDepartment of Pathology, University of Veterinary Medicine Hannover, Foundation, Hannover, GermanyCenter for Systems Neuroscience, Hannover Graduate School for Neurosciences, Infection Medicine, and Veterinary Sciences (HGNI), Hannover, GermanyResearch Center for Emerging Infections and Zoonoses, University of Veterinary Medicine Hannover, Foundation, Hannover, GermanyDepartment of Pathology, University of Veterinary Medicine Hannover, Foundation, Hannover, GermanyResearch Center for Emerging Infections and Zoonoses, University of Veterinary Medicine Hannover, Foundation, Hannover, GermanyDivision of Virology, Institute for Infectious Diseases and Zoonoses, Ludwig Maximilian University Munich, Munich, GermanyGerman Center for Infection Research (DZIF), partner site Munich, Munich, GermanyDepartment of Pathology, University of Veterinary Medicine Hannover, Foundation, Hannover, GermanyCenter for Systems Neuroscience, Hannover Graduate School for Neurosciences, Infection Medicine, and Veterinary Sciences (HGNI), Hannover, GermanyResearch Center for Emerging Infections and Zoonoses, University of Veterinary Medicine Hannover, Foundation, Hannover, GermanyDivision of Virology, Institute for Infectious Diseases and Zoonoses, Ludwig Maximilian University Munich, Munich, GermanyGerman Center for Infection Research (DZIF), partner site Munich, Munich, GermanyResearch Center for Emerging Infections and Zoonoses, University of Veterinary Medicine Hannover, Foundation, Hannover, GermanyResearch Center for Emerging Infections and Zoonoses, University of Veterinary Medicine Hannover, Foundation, Hannover, GermanyIntroductionTick-borne encephalitis virus (TBEV) is an important human pathogen that can cause a serious disease involving the central nervous system (tick-borne encephalitis, TBE). Although approved inactivated vaccines are available, the number of TBE cases is rising, and breakthrough infections in fully vaccinated subjects have been reported in recent years.MethodsIn the present study, we generated and characterized a recombinant Modified Vaccinia virus Ankara (MVA) for the delivery of the pre-membrane (prM) and envelope (E) proteins of TBEV (MVA-prME).ResultsMVA-prME was tested in mice in comparison with a licensed vaccine FSME-IMMUN® and proved to be highly immunogenic and afforded full protection against challenge infection with TBEV.DiscussionOur data indicate that MVA-prME holds promise as an improved next-generation vaccine for the prevention of TBE.https://www.frontiersin.org/articles/10.3389/fimmu.2023.1182963/fullTBEVMVAFSME-IMMUN®protectionvaccinationvirus-neutralizing antibodies |
| spellingShingle | Mareike Kubinski Jana Beicht Isabel Zdora Isabel Zdora Jeannine Biermann Christina Puff Thomas Gerlach Alina Tscherne Alina Tscherne Wolfgang Baumgärtner Wolfgang Baumgärtner Albert D. M. E. Osterhaus Gerd Sutter Gerd Sutter Chittappen Kandiyil Prajeeth Guus F. Rimmelzwaan A recombinant Modified Vaccinia virus Ankara expressing prME of tick-borne encephalitis virus affords mice full protection against TBEV infection Frontiers in Immunology TBEV MVA FSME-IMMUN® protection vaccination virus-neutralizing antibodies |
| title | A recombinant Modified Vaccinia virus Ankara expressing prME of tick-borne encephalitis virus affords mice full protection against TBEV infection |
| title_full | A recombinant Modified Vaccinia virus Ankara expressing prME of tick-borne encephalitis virus affords mice full protection against TBEV infection |
| title_fullStr | A recombinant Modified Vaccinia virus Ankara expressing prME of tick-borne encephalitis virus affords mice full protection against TBEV infection |
| title_full_unstemmed | A recombinant Modified Vaccinia virus Ankara expressing prME of tick-borne encephalitis virus affords mice full protection against TBEV infection |
| title_short | A recombinant Modified Vaccinia virus Ankara expressing prME of tick-borne encephalitis virus affords mice full protection against TBEV infection |
| title_sort | recombinant modified vaccinia virus ankara expressing prme of tick borne encephalitis virus affords mice full protection against tbev infection |
| topic | TBEV MVA FSME-IMMUN® protection vaccination virus-neutralizing antibodies |
| url | https://www.frontiersin.org/articles/10.3389/fimmu.2023.1182963/full |
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