Enhanced Characterization of Lysine-Linked Antibody Drug Conjugates Enabled by Middle-Down Mass Spectrometry and Higher-Energy Collisional Dissociation-Triggered Electron-Transfer/Higher-Energy Collisional Dissociation and Ultraviolet Photodissociation

Enhanced Characterization of Lysine-Linked Antibody Drug Conjugates Enabled by Middle-Down Mass Spectrometry and Higher-Energy Collisional Dissociation-Triggered Electron-Transfer/Higher-Energy Collisional Dissociation and Ultraviolet Photodissociation

As the development of new biotherapeutics advances, increasingly sophisticated tandem mass spectrometry methods are needed to characterize the most complex molecules, including antibody drug conjugates (ADCs). Lysine-linked ADCs, such as trastuzumab-emtansine (T-DM1), are among the most heterogeneou...

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Bibliographic Details
Main Authors: Eleanor Watts, Aarti Bashyal, Sean D. Dunham, Christopher M. Crittenden, Jennifer S. Brodbelt
Format: Article
Language:English
Published: MDPI AG 2024-04-01
Series:Antibodies
Subjects:
antibody drug conjugate
trastuzumab-emtansine
tandem mass spectrometry
payload
biotherapeutic
Online Access:https://www.mdpi.com/2073-4468/13/2/30

Internet

https://www.mdpi.com/2073-4468/13/2/30

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