Induction of neutralizing antibodies in mice immunized with scorpion toxins detoxified by liposomal entrapment
The possibility of producing neutralizing antibodies against the lethal effects of scorpion toxins was evaluated in the mouse model by immunization with an immunogen devoid of toxicity. A toxic fraction (5 mg) from the venom of the scorpion Tityus serrulatus was entrapped in sphingomyelin-cholestero...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Associação Brasileira de Divulgação Científica
1997-07-01
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| Series: | Brazilian Journal of Medical and Biological Research |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997000700010 |
| _version_ | 1828485692165980160 |
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| author | S.G. Fonseca A.M.M. Ferreira C.R. Diniz C. Chávez-Olórtegui |
| author_facet | S.G. Fonseca A.M.M. Ferreira C.R. Diniz C. Chávez-Olórtegui |
| author_sort | S.G. Fonseca |
| collection | DOAJ |
| description | The possibility of producing neutralizing antibodies against the lethal effects of scorpion toxins was evaluated in the mouse model by immunization with an immunogen devoid of toxicity. A toxic fraction (5 mg) from the venom of the scorpion Tityus serrulatus was entrapped in sphingomyelin-cholesterol liposomes. The liposomes were treated for 1 h at 37oC with a 1% (w/w) trypsin solution in 0.2 M sodium carbonate buffer, pH 8.3. This treatment led to a strong reduction in venom toxicity. Immunization was performed as follows: mice were injected sc with 20 µg of the liposome-entrapped toxic fraction on days 1 and 21 and a final injection (20 µg) was administered ip on day 36. After injection of the immunogen, all mice developed an IgG response which was shown to be specific for the toxic antigen. The antibodies were measured 10 days after the end of the immunization protocol. In an in vitro neutralization assay we observed that pre-incubation of a lethal dose of the toxic fraction with immune serum strongly reduced its toxicity. In vivo protection assays showed that mice with anti-toxin antibodies could resist the challenge with the toxic fraction, which killed, 30 min after injection, all non-immune control mice |
| first_indexed | 2024-12-11T09:19:14Z |
| format | Article |
| id | doaj.art-f30e26bfd8484661939382db85ec27ef |
| institution | Directory Open Access Journal |
| issn | 0100-879X 1414-431X |
| language | English |
| last_indexed | 2024-12-11T09:19:14Z |
| publishDate | 1997-07-01 |
| publisher | Associação Brasileira de Divulgação Científica |
| record_format | Article |
| series | Brazilian Journal of Medical and Biological Research |
| spelling | doaj.art-f30e26bfd8484661939382db85ec27ef2022-12-22T01:13:18ZengAssociação Brasileira de Divulgação CientíficaBrazilian Journal of Medical and Biological Research0100-879X1414-431X1997-07-0130788310.1590/S0100-879X1997000700010Induction of neutralizing antibodies in mice immunized with scorpion toxins detoxified by liposomal entrapmentS.G. FonsecaA.M.M. FerreiraC.R. DinizC. Chávez-OlórteguiThe possibility of producing neutralizing antibodies against the lethal effects of scorpion toxins was evaluated in the mouse model by immunization with an immunogen devoid of toxicity. A toxic fraction (5 mg) from the venom of the scorpion Tityus serrulatus was entrapped in sphingomyelin-cholesterol liposomes. The liposomes were treated for 1 h at 37oC with a 1% (w/w) trypsin solution in 0.2 M sodium carbonate buffer, pH 8.3. This treatment led to a strong reduction in venom toxicity. Immunization was performed as follows: mice were injected sc with 20 µg of the liposome-entrapped toxic fraction on days 1 and 21 and a final injection (20 µg) was administered ip on day 36. After injection of the immunogen, all mice developed an IgG response which was shown to be specific for the toxic antigen. The antibodies were measured 10 days after the end of the immunization protocol. In an in vitro neutralization assay we observed that pre-incubation of a lethal dose of the toxic fraction with immune serum strongly reduced its toxicity. In vivo protection assays showed that mice with anti-toxin antibodies could resist the challenge with the toxic fraction, which killed, 30 min after injection, all non-immune control micehttp://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997000700010liposomesneutralizing antibodiesTityus serrulatusimmunizationtoxins |
| spellingShingle | S.G. Fonseca A.M.M. Ferreira C.R. Diniz C. Chávez-Olórtegui Induction of neutralizing antibodies in mice immunized with scorpion toxins detoxified by liposomal entrapment Brazilian Journal of Medical and Biological Research liposomes neutralizing antibodies Tityus serrulatus immunization toxins |
| title | Induction of neutralizing antibodies in mice immunized with scorpion toxins detoxified by liposomal entrapment |
| title_full | Induction of neutralizing antibodies in mice immunized with scorpion toxins detoxified by liposomal entrapment |
| title_fullStr | Induction of neutralizing antibodies in mice immunized with scorpion toxins detoxified by liposomal entrapment |
| title_full_unstemmed | Induction of neutralizing antibodies in mice immunized with scorpion toxins detoxified by liposomal entrapment |
| title_short | Induction of neutralizing antibodies in mice immunized with scorpion toxins detoxified by liposomal entrapment |
| title_sort | induction of neutralizing antibodies in mice immunized with scorpion toxins detoxified by liposomal entrapment |
| topic | liposomes neutralizing antibodies Tityus serrulatus immunization toxins |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997000700010 |
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