Identification of Novel Endogenous Controls for qPCR Normalization in SK-BR-3 Breast Cancer Cell Line

Identification of Novel Endogenous Controls for qPCR Normalization in SK-BR-3 Breast Cancer Cell Line

Normalization of gene expression using internal controls or reference genes (RGs) has been the method of choice for standardizing the technical variations in reverse transcription quantitative polymerase chain reactions (RT-qPCR). Conventionally, <i>ACTB</i> and <i>GAPDH</i>...

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Bibliographic Details
Main Authors: Nityanand Jain, Ingrida Mitre, Dina Nitisa, Valdis Pirsko, Inese Cakstina-Dzerve
Format: Article
Language:English
Published: MDPI AG 2021-10-01
Series:Genes
Subjects:
SK-BR-3
RT-qPCR
reference genes
hypoxia
gene expression
breast cancer cell line
Online Access:https://www.mdpi.com/2073-4425/12/10/1631

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https://www.mdpi.com/2073-4425/12/10/1631

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