Improvement of Rotavirus Genotyping Method by Using the Semi-Nested Multiplex-PCR With New Primer Set

Improvement of Rotavirus Genotyping Method by Using the Semi-Nested Multiplex-PCR With New Primer Set

Rotavirus A (RVA) is a major cause of gastroenteritis in infants and young children. After vaccine introduction, RVA surveillance has become more important for monitoring changes in genotype distribution, and the semi-nested multiplex-PCR is a popular method for RVA genotyping. In particular, the VP...

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Bibliographic Details
Main Authors: Yoshiki Fujii, Yen Hai Doan, Rury Mega Wahyuni, Maria Inge Lusida, Takako Utsumi, Ikuo Shoji, Kazuhiko Katayama
Format: Article
Language:English
Published: Frontiers Media S.A. 2019-03-01
Series:Frontiers in Microbiology
Subjects:
rotavirus
genotyping
nested-PCR
multiplex-PCR
primer design
equine-like G3
Online Access:https://www.frontiersin.org/article/10.3389/fmicb.2019.00647/full

Internet

https://www.frontiersin.org/article/10.3389/fmicb.2019.00647/full

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